Addgene

We narrowed to 2,326 results for: EFS

Showing: 921 - 960 of 2326 results

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pEGFPm-DAD

Plasmid

#25410

Depositor

Arthur Alberts

Article

Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

Insert

Diap3 (Diaph3 Mouse)

Use

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

mDia2 Amino Acids 1031-1171; Q1091P mutation

Promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCoofy50

Plasmid

#55189

Purpose

Mammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

8x His, FLAG, HA, PreScission site (3C protease s…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID3S

Plasmid

#188651

Purpose

Expresses SaAID3S and gRNA in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

nSaCas9-AID3S--UGI
Sa-sgRNA

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

EF-1a and H1

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID2S

Plasmid

#188652

Purpose

Expresses SaAID2S and gRNA in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S-nSaCas9--UGI
Sa-sgRNA

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

EF-1a and H1

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSκ

Plasmid

#68291

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 4xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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p3PA-Puro-iTk

Plasmid

#109067

Purpose

inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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C-12-BE

Plasmid

#135040

Purpose

C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L2_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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N-8-BE

Plasmid

#135023

Purpose

C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C-1-BE

Plasmid

#135029

Purpose

C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

PmCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSγ

Plasmid

#68284

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
N-4-BE

Plasmid

#135019

Purpose

C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSε

Plasmid

#68286

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 1xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSθ

Plasmid

#68289

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSν

Plasmid

#68294

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 5xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSβ

Plasmid

#68283

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 1xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 EF1a-EGFP

Plasmid

#231925

Purpose

3rd generation lentiviral transfer plasmid with EGFP reporter gene

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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MCP-YPet

Plasmid

#215747

Purpose

Expresses MS2 coat protein (MCP) fused to YPet

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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C-13-BE

Plasmid

#135041

Purpose

C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C-7-BE

Plasmid

#135035

Purpose

C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C-6-BE

Plasmid

#135034

Purpose

C-T base editing using LjDA1L2_2 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjDA1L2_2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C-11-BE

Plasmid

#135039

Purpose

C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L2_2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSη

Plasmid

#68288

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 2xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSζ

Plasmid

#68287

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSδ

Plasmid

#68285

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 1xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiPVe3_dTomato_nlsdTomato

Plasmid

#213940

Purpose

AAV construct expressing dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WP0003_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiSSTe10_dTomato_nlsdTomato

Plasmid

#213814

Purpose

AAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiSSTe4_dTomato_nlsdTomato

Plasmid

#213944

Purpose

AAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: AAV_WDS0004_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiVIPe4_dTomato_nlsdTomato

Plasmid

#213855

Purpose

AAV construct expressing dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiCHATe27_dTomato_nlsdTomato

Plasmid

#213829

Purpose

AAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiLAMP5e3_dTomato_nlsdTomato

Plasmid

#218792

Purpose

AAV construct with dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiPVe4_dTomato_nlsdTomato

Plasmid

#213936

Purpose

AAV construct expressing dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiPVe4_ChR2_mCherry

Plasmid

#213937

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Has Service

AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiPVe3_Gq_dTomato

Plasmid

#213942

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

HA-GqDREADD-dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiSSTe4_GCaMP6f

Plasmid

#213947

Purpose

AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiPVe4_eGFP

Plasmid

#221463

Purpose

AAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiCHATe27_Gq_dTomato

Plasmid

#213832

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

HA-GqDREADD-dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiPVe3_GCaMP6f

Plasmid

#213943

Purpose

AAV construct expressing GCaMP6f driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_GCaMP6f

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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