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Showing: 1151 - 1200 of 17736 results

  2. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid
    #236245
    Purpose
    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    Use
    AAV
    Tags
    mTagBFP2
    Promoter
    U6 and human Synapsin1
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236245-plasmid-map-sequence-id-471239

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  4. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

    Plasmid
    #176087
    Purpose
    EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Lys72 to Ala, and mutation in the PAM…
    Promoter
    CMV
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    176087-plasmid-map-sequence-id-352865

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  6. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  7. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

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  8. pTO_CHMP2B_LAP

    Plasmid
    #101848
    Purpose
    Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Flp-frt recombination, tet-inducible expression
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    silent mutation in Thr 104; A to T
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101848-plasmid-map-sequence-id-200235

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  9. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

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  10. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

    Plasmid
    #176143
    Purpose
    EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Arg65 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176143-plasmid-map-sequence-id-353151

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  11. pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro

    Plasmid
    #176089
    Purpose
    EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutations in Lys35 to Ala, Lys68 to Ala, and Lys7…
    Promoter
    CMV
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176089-plasmid-map-sequence-id-352910

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  13. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid
    #176139
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176139-plasmid-map-sequence-id-353012

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  16. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  17. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid
    #176140
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176140-plasmid-map-sequence-id-353035

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  19. pT7-module1

    Plasmid
    #222838
    Purpose
    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222838-plasmid-map-sequence-id-446490

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  20. pT7-module2

    Plasmid
    #222839
    Purpose
    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222839-plasmid-map-sequence-id-446492

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  21. pT7-module3

    Plasmid
    #222840
    Purpose
    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 3)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222840-plasmid-map-sequence-id-446494

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  22. pT7-module4

    Plasmid
    #222841
    Purpose
    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 4)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222841-plasmid-map-sequence-id-446496

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  23. pT7-module5

    Plasmid
    #222842
    Purpose
    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 5)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222842-plasmid-map-sequence-id-446497

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  24. pT7-module6

    Plasmid
    #222843
    Purpose
    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 6)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222843-plasmid-map-sequence-id-446499

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  25. pT7-module1*5

    Plasmid
    #222844
    Purpose
    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1*5)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222844-plasmid-map-sequence-id-446502

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  26. pT7-module2*5

    Plasmid
    #222845
    Purpose
    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2*5)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222845-plasmid-map-sequence-id-446504

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  27. pAAV Syn-V5-TurboID-CAAX_v1

    Plasmid
    #250175
    Purpose
    Membrane-TurboID variant for neuron expression
    Depositor
    Christina Kim
    Insert
    TurboID
    Use
    AAV
    Tags
    CAAX and V5
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250175-plasmid-map-sequence-id-499109

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  28. pAAV Syn-V5-TurboID-CAAX_v2

    Plasmid
    #250176
    Purpose
    Membrane-TurboID variant for neuron expression
    Depositor
    Christina Kim
    Insert
    TurboID
    Use
    AAV
    Tags
    CAAX and V5
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250176-plasmid-map-sequence-id-499068

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  29. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

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  30. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

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  31. pAG471 Lyn11-iFAST

    Plasmid
    #130817
    Purpose
    Expresses Lyn11-iFAST in mammalian cells (inner plasma membrane labeling)
    Depositor
    Arnaud Gautier
    Insert
    Lyn11-iFAST
    Tags
    myc-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130817-plasmid-map-sequence-id-254955

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  35. pET28-GG-PUF

    Plasmid
    #52137
    Purpose
    Receiving vector for PUM-HD assembly and expression in E.coli
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Type
    Empty backbone
    Tags
    PUM-HD flanking region 1' and PUM-HD flankin…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    52137-plasmid-map-sequence-id-80978

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  40. pMSP1D1_sfCherryL

    Plasmid
    #248924
    Purpose
    Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfCherry for nanodisc formation (longer linker)
    Depositor
    Michael Marty
    Article
    Cleveland et al bioRxiv 2026.04.07.716332
    Insert
    MSP1D1_sfCherryL
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 6, 2026
    Availability
    Industry, Academic Institutions, and Nonprofits
    248924-plasmid-map-sequence-id-496203

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  41. pMSP1D1_sfCherry

    Plasmid
    #248923
    Purpose
    Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfCherry for nanodisc formation
    Depositor
    Michael Marty
    Article
    Cleveland et al bioRxiv 2026.04.07.716332
    Insert
    MSP1D1_sfCherry
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 6, 2026
    Availability
    Industry, Academic Institutions, and Nonprofits
    248923-plasmid-map-sequence-id-496202

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  42. pMSP1D1_HaloTag

    Plasmid
    #248926
    Purpose
    Bacterial expression of Halo-tagged membrane scaffold protein MSP1D1 fused to HaloTag for nanodisc formation
    Depositor
    Michael Marty
    Article
    Cleveland et al bioRxiv 2026.04.07.716332
    Insert
    MSP1D1_HaloTag
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248926-plasmid-map-sequence-id-495593

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  44. pMSP1D1_sfGFP

    Plasmid
    #248920
    Purpose
    Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfGFP for nanodisc formation
    Depositor
    Michael Marty
    Article
    Cleveland et al bioRxiv 2026.04.07.716332
    Insert
    MSP1D1_sfGFP
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    248920-plasmid-map-sequence-id-494374

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  50. pCGFP-EU

    Plasmid
    #161968
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Tags
    EGFP His8
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    161968-plasmid-map-sequence-id-319729

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Showing: 1151 - 1200 of 17736 results