We narrowed to 12,150 results for: 112

1. pcDNA3.1-hRIPK1-Flag-K377R

   Plasmid

   #112489

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K377R

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSNAI2-donor

   Plasmid

   #112378

   Purpose

   CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP

   Depositor

   Kevin White

   Insert

   SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVHC

   Plasmid

   #112614

   Purpose

   promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Venus (H2BC3 Human, Aequorea victoria)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Promoterless vector, ready for promoter for mamma…

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   No Promoter

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CD4-C-BFP HDRT Source (pTR 176)

   Plasmid

   #112020

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-BFP HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

   Plasmid

   #112211

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   C-terminus of dCas9
   

   Use

   AAV

   Mutation

   D10A

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. gRNAs[bTub+Sxl].1046B

   Plasmid

   #112692

   Purpose

   express two gRNA targeting bTub & Sxl under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
   

   Use

   CRISPR

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5 GST MetaP2

   Plasmid

   #112750

   Purpose

   express HA-GST-FRB-MetaP2

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   HA-GST-FRB-MetaP2 (METAP2 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. rd-his

   Plasmid

   #112911

   Purpose

   Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag

   Depositor

   C. James McKnight

   Article

   Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

   Insert

   dematin (DMTN Human)
   

   Tags

   6-his tag, Glutathione-S-Transferase, and preciss…

   Expression

   Bacterial

   Mutation

   89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…

   Promoter

   T7

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM1157

   Plasmid

   #112534

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   luxCDABE
   

   Expression

   Bacterial

   Promoter

   PL(HrtO) and ProD

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSopBC460S

    Plasmid

    #112514

    Purpose

    PsopB driven sopB (catalytically inactive) and sigE

    Depositor

    Olivia Steele-Mortimer

    Article

    Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

    Insert

    sopB (catalytically inactive)/sigE operon
    

    Expression

    Bacterial

    Mutation

    SopB C460S

    Promoter

    PsopB

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGex4t2 with 244-458AA SUUR fragment

    Plasmid

    #112989

    Purpose

    Expresses fusion of GST and amino acids 244-458 of Drosophila SUUR

    Depositor

    Terry Orr-Weaver

    Article

    Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

    Insert

    SuUR (SuUR Fly)
    

    Expression

    Bacterial

    Mutation

    amino acids 244-458

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOmpT

    Plasmid

    #112123

    Purpose

    Bacterial expression outer membrane protein T (OmpT), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpT (ompT E. coli)
    

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. rag1 long

    Plasmid

    #11299

    Depositor

    Lisa Steiner

    Article

    Willett et al Immunogenetics. 1997 . 45(6):394-404.

    Insert

    rag1 (rag1 Zebrafish)
    

    Expression

    Bacterial

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid

    #112029

    Purpose

    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Insert

    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    

    Tags

    Split mCherry

    Expression

    Bacterial

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTORKm43GW

    Plasmid

    #112897

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418).

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

    Type

    Empty backbone

    Use

    Oomycete expression

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE human IRS-1 CAAX

    Plasmid

    #11237

    Depositor

    Ronald Kahn

    Article

    Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    C-terminal prenylation motif makes IRS-1 localize…

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

17. rat APOBEC1-EGFP

    Plasmid

    #112857

    Purpose

    plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

    Depositor

    Silvestro Conticello

    Article

    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

    Insert

    apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTEAD1-donor

    Plasmid

    #112329

    Purpose

    CRISPR donor plasmid to tag human transcription factor TEAD1 with GFP

    Depositor

    Kevin White

    Insert

    TEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRNATU6.1 HDAC3 shRNA2

    Plasmid

    #112271

    Purpose

    HDAC3 KD

    Depositor

    Simone Di Giovanni

    Insert

    shRNA against mouse HDAC3 (Hdac3 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. bTub-GFP.766A

    Plasmid

    #112695

    Purpose

    expressed GFP in testes, under bTub promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    bTub-GFP-SV40
    

    Expression

    Insect

    Promoter

    bTub

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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