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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pH6-spTorA-mCherry

Plasmid

#169040

Purpose

mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

H6-spTorA-mCherry

Use

Tags

6xHis and spTorA (signal peptide of E. coli TorA)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:GFP1–10

Plasmid

#125663

Purpose

Binary vector for the inducible expression of GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

GFP1–10

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

UBQ10:sXVE:

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAd5-B7-deltaE4

Plasmid

#122560

Purpose

Modified block 7, with deletion in E4

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 30888-35938

Use

Synthetic Biology

Tags

Expression

Mutation

Adenovirus sequences 32874-35461 replaced with Sp…

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-GAL4DBD

Plasmid

#26256

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

ZIP- Gal4DBD (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP

Plasmid

#84256

Purpose

Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-QF2

Plasmid

#122563

Purpose

Integration of QF2 sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-QF2-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMCSG53_Nsp1

Plasmid

#167256

Purpose

Bacterial expression of Nsp1 residues 13-127

Depositor

Alexei Savchenko

Article

Semper et al iScience. 2021 Jan 22;24(1):101903. doi:

Insert

Nsp1 (residues 13-127) (ORF1ab SARS-CoV-2)

Use

Tags

His6-TEV site

Expression

Bacterial

Mutation

none

Promoter

Available since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pN_35S/mApple

Plasmid

#132566

Purpose

mApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mApple

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-Cre

Plasmid

#122562

Purpose

Integration of Cre sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-Cre-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA, TadA*E59A)

Plasmid

#125662

Purpose

pCMV-ABEmax(TadA, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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FnR9-10

Plasmid

#182567

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Use

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1379A

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan 12. ():.

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mutation

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pLK27

Plasmid

#37256

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

Scribble (SCRIB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1197-1630

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pET-s34T

Plasmid

#28256

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain H3-H4 with Thrombin site

Use

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between H3 and H4

Promoter

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pCMV myc DAF-12

Plasmid

#32566

Depositor

Al Fisher

Article

Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

Insert

DAF-12 (daf-12 Nematode)

Use

Tags

myc epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:ER-GFP1–10

Plasmid

#125664

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-GFP1–10

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

UBQ10:sXVE:

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUCBB-lacZa

Plasmid

#32563

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

LacZ-alpha

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2

Plasmid

#79256

Purpose

Expresses EGFP-tagged DmPAN2 in S2 cells

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

DmPAN2 (PAN2 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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