Addgene

We narrowed to 8,550 results for: ef1

Showing: 461 - 480 of 8550 results

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pLenti_Ef1a:PE2-P2A-hMLH1dn

Plasmid

#184444

Purpose

Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Ef1A

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-FAKSPARK2

Plasmid

#195265

Purpose

express wtFAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

FAKSPARK2 sensor

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

ef1a

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PTEF1-yEGFPCLN2PEST-pRS406

Plasmid

#64406

Purpose

TEF promoter driven GFP expression

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

TEF1 promoter (TEF1 Budding Yeast)

Use

Tags

yEGFPCLN2PEST (destabilized yeast EGFP)

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-vCre

Plasmid

#55638

Purpose

EF1a-driven expression of VCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Insert

vCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-2A-ZsGreen

Plasmid

#231985

Purpose

Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

2A-ZsGreen

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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mc2 puro EF1a

Plasmid

#206221

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-mutFAKSPARK2

Plasmid

#195266

Purpose

express Tyr to Phe mutant FAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

Tyr to Phe mutant FAKSPARK2 sensor

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

ef1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-Bmi1

Plasmid

#31783

Depositor

Xin Chen

Article

Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

Insert

Bmi1 (BMI1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP

Plasmid

#32628

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Sce

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Bamh1

Plasmid

#203308

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-ZsGreen1_Puro

Plasmid

#192661

Purpose

3rd generation lenti vector encoding ZsGreen1 with 2A Puro resistance marker

Depositor

Jeffrey Miner

Insert

ZsGreen1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEF1-alpha-IIb

Plasmid

#27288

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha IIb (ITGA2B Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

n/a

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EF1α-EGFP

Plasmid

#21320

Purpose

Control Lentiviral vector with strong EF1a promoter, expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

Plasmid

#174896

Purpose

Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pVH21-Tier1-PhEF1a

Plasmid

#169561

Purpose

Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-CCNE2

Plasmid

#162554

Purpose

Overexpression of CCNE2

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of CCNE2 (CCNE2 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a_flagLaM4-Notch-Gal4VP64

Plasmid

#216669

Purpose

Expresses anti-mCherry synNotch Gal4VP64

Depositor

Leonardo Morsut

Insert

anti-mCherry synNotch Gal4VP64

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iDreV

Plasmid

#140136

Purpose

can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iDreV

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Nluc

Plasmid

#73024

Purpose

Express Nluc reporter under EF1 promoter

Depositor

Kazuhiro Oka

Insert

Nluc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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