We narrowed to 5,415 results for: PEP;

1. pCEP4-myc-EphB2

   Plasmid

   #200982

   Purpose

   Mammalian expression plasmid for myc-tagged EphB2

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB2 (EPHB2 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CXCR6-DuET

   Plasmid

   #213223

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CXCR6 (CXCR6 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-miniDi-P2A-mCherry

   Plasmid

   #204358

   Purpose

   AAV vector for miniDi expression under the control of human synapsin promoter

   Depositor

   Masao Doi

   Article

   Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

   Insert

   miniDi-P2A-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   The third intracellular loop (ICL3) of hM4Di was …

   Promoter

   hSyn

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166014

   Purpose

   mammalian expression of HCoV-HKU1 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   HCoV-HKU1 S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

   Plasmid

   #194457

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-CD19 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HRH1-DuET

   Plasmid

   #213312

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HRH1 (HRH1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CNR2-DuET

   Plasmid

   #213214

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CNR2 (CNR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CXCR2-DuET

   Plasmid

   #213219

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CXCR2 (CXCR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-miniDq-P2A-mCherry

   Plasmid

   #204357

   Purpose

   AAV vector for miniDq expression under the control of human synapsin promoter

   Depositor

   Masao Doi

   Article

   Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

   Insert

   miniDq-P2A-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   The third intracellular loop (ICL3) of hM3Dq was …

   Promoter

   hSyn

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ADRB3-DuET

    Plasmid

    #213182

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADRB3 (ADRB3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GPR143-DuET

    Plasmid

    #213264

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR143 (GPR143 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166013

    Purpose

    mammalian expression of MERS-CoV soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    MERS-CoV S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BDKBR2-DuET

    Plasmid

    #213190

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    BDKBR2 (BDKRB2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C3AR1-DuET

    Plasmid

    #213191

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    C3AR1 (C3AR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SpySwitch

    Plasmid

    #184225

    Purpose

    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpySwitch
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pStr-KDEL_TPV-CXCL9-mCherry-SBP

    Plasmid

    #170677

    Purpose

    Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Inserts

    CXCL9 (CXCL9 Human, Synthetic)
    streptavidin-KDEL
    

    Use

    Tags

    SBP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and T7

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-(BbsI)_CBh-Cas9-T2A-BFP

    Plasmid

    #64323

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-FLAG-TAPBPR

    Plasmid

    #135500

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    Luminal FLAG epitope tag and Signal peptide from …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166012

    Purpose

    mammalian expression of SARS-CoV soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-26b-NbVHH05-HA-His

    Plasmid

    #171567

    Purpose

    Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-HA-His (UBE2J1 Human)
    

    Use

    Tags

    HA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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