We narrowed to 34,203 results for: CaS

1. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

   Plasmid

   #171101

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Olig2 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124287

   Purpose

   sgRNA against human Col7A1

   Depositor

   Katalin Josvay, Csaba Vizler

   Insert

   Collagen type VII alpha 1 chain (COL7A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

   Plasmid

   #171102

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Otx2 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120294

   Purpose

   AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   C-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   split-intein

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

   Plasmid

   #121819

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

   Plasmid

   #101236

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K918A/V922A/R925A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K918A, V922A, R925A and Q926A

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126777

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124288

   Purpose

   sgRNA against human NFATc3

   Depositor

   Katalin Josvay, Csaba Vizler

   Insert

   NFATc3 nuclear factor of activated T cells (NFATC3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

9. Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124286

   Purpose

   sgRNA against human Col7A1

   Depositor

   Katalin Josvay, Csaba Vizler

   Insert

   Collagen type VII alpha 1 chain (COL7A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Plasmid

    #101235

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant F491A/M495A/T496A/N497A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    F491A, M495A, T496A, N497A and Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Plasmid

    #101234

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant T657A/G658A/W659A/R661A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    T657A, G658A, W659A, R661A and Q926A

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid

    #101220

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A and Q926A

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid

    #101232

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G528A, V583A, E584A, D585A, N588A and Q926A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236376

    Purpose

    Overexpression of HyperdFnCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdFnCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E184R, T279R, D340R, D409R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

    Plasmid

    #236368

    Purpose

    Overexpression of HyperdLbCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D156R, D235R, E292R, D350R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236375

    Purpose

    Overexpression of dFnCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    dFnCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236363

    Purpose

    Overexpression of endAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    endAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E174R, S542R, K548R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236362

    Purpose

    Overexpression of HyperdAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E174R, D256R, E321R, E371R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236361

    Purpose

    Overexpression of dAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    dAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRCA422 - pHR-UCOE-SFFV-VPH-dcas9-p2a-mCherry

    Plasmid

    #238165

    Purpose

    Express VPH-dCas9-P2A-mCherry in mammalian cells. Lentiviral

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet (2025)

    Insert

    VPH-dCas9-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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