We narrowed to 10,545 results for: Uty

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Expression

Bacterial

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9.mCherry

Plasmid

#208342

Purpose

Lentivirus with EF1a driven Cas9 linked (P2A) to mCherry.

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Inserts

Cas9
mCherry

Use

CRISPR and Lentiviral

Tags

nuclear localization sequence (NLS) and FLAG tag

Promoter

EF-1a

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pAcBac1-GFP(39TAG)

Plasmid

#217360

Purpose

Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

Enhanced green fluorescent protein

Use

Baculoviral

Expression

Insect and Mammalian

Mutation

Y39TAG

Promoter

CMV

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmExRai-AKAR2

Plasmid

#161755

Purpose

Plasma membrane-targeted ExRai-AKAR2.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

Lyn-ExRai-AKAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-erExRai-AKAR2

Plasmid

#161756

Purpose

ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

CYP450-ExRai-AKAR2

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSIN-PAmCherry-KFERQ-NE

Plasmid

#102365

Purpose

Lentiviral overexpression of PA-mCherry-KFERQ fusion

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Inserts

PA-mCherry
KFERQ peptide

Use

Lentiviral

Tags

NE

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C-ABE8e

Plasmid

#185667

Purpose

Expresses eNme2-C-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-C

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-TadCBEd-ivt-template

Plasmid

#193843

Purpose

Template for in vitro transcription of TadCBEd (with SpCas9)

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd with SpCas9

Use

In vitro transcription template

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

Plasmid

#40810

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(31-51), S218E, S222D

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pDB1 (CK2alpha)

Plasmid

#27083

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-Gal4UAS-GFP-PGK-BFP

Plasmid

#247506

Purpose

GAL4/UAS expression of GFP and constitutive expression of BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

GAL4/UAS expression of GFP and constitutive expression of BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MCS-GFP-SV-puro

Plasmid

#73582

Purpose

Third generation lentiviral vector expressing GFP and puromycin

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

GFP tag

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF0

Plasmid

#200383

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF1

Plasmid

#200384

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pHS2056

Plasmid

#239837

Purpose

OMEGAOff + omegaRNA scaffold AAV expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold

Use

AAV

Tags

HA and SV40 NLS

Mutation

Insertion of REC domain from NbaCas9-1 + E137K/E4…

Promoter

EFS; human U6

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF2

Plasmid

#200385

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1725

Plasmid

#105870

Purpose

High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

iCreERT2-FFrt-KanR-FFrt

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd

Plasmid

#193835

Purpose

Express TadCBEd (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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