We narrowed to 24,865 results for: nes

Showing: 4781 - 4800 of 24865 results

Results per page:

Sort results by:

MSCV p2GM AMPK alpha2hp1 alpha1hp1

Plasmid

#89492

Purpose

shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

2 hairpins against mouse/human AMPKalpha1 and alpha2

Use

Retroviral

Expression

Mammalian

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
DYR1A_HUMAN_D0

Plasmid

#79690

Purpose

This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK1A (DYRK1A Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-PHFF

Plasmid

#100280

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)

Tags

FLAG, T7 and Mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

All genes are codon-optimized for expression in h…

Promoter

CAG promoter

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AURKA_HUMAN_D0

Plasmid

#79739

Purpose

This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AURKA (AURKA Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pEN_TT 3XFLAG-wtGATA6-3XAU1

Plasmid

#72612

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight promoter

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

Plasmid

#187954

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-hHDAC4-tagBFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pC-SUR-YR

Plasmid

#61768

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Sur gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

Magnaporthe oryzae ILV promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-(BsaI)_CBh-Cas9-T2A-mCherry

Plasmid

#135012

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

3x Flag, 3xFLAG (N terminal on insert), NLS, and …

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
NEK1_HUMAN_D0

Plasmid

#79725

Purpose

This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK1 (NEK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
LMPd Ametrine Got2 shRNA#3

Plasmid

#220593

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got2 shRNA (Got2 Mouse)

Use

Retroviral

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MERTK_HUMAN_D0

Plasmid

#79705

Purpose

This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MERTK (MERTK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Bxb1Donor_WT_ADRB2_LucReporter

Plasmid

#129788

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Promoter

N/A

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MARK3_HUMAN_D0

Plasmid

#79740

Purpose

This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MARK3 (MARK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

Plasmid

#101230

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EPHB1_HUMAN_D0

Plasmid

#79694

Purpose

This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB1 (EPHB1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK3_HUMAN_D0

Plasmid

#79689

Purpose

This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK3 (STK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2G_HUMAN_D0

Plasmid

#79711

Purpose

This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2G (CAMK2G Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MEF2A-Sensor

Plasmid

#208928

Purpose

in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

Mef2a docking sequence+Phospho site (MEF2A Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge