We narrowed to 11,748 results for: pcDNA

1. pcDNA5/FRT/TO_Lyn11-STf

   Plasmid

   #182008

   Purpose

   CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   Lyn11-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3582 pcDNA3 Bad S136E

   Plasmid

   #8800

   Depositor

   Stanley Korsmeyer

   Article

   Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

   Insert

   Bad (Bad Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S136E

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-HA-RIPK3 2KR(55,363)

   Plasmid

   #78811

   Purpose

   To overexpress RIPK3 2KR(55,363) in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K55R, K363R

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-DDX11-Flag-KAE

   Plasmid

   #120728

   Purpose

   Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

   Depositor

   Francesca Pisani

   Article

   Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

   Insert

   DDX11 KAE mutant (DDX11 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Substitution of E201 and Y202 with K and A

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_HLA-cmyc-GRM3 C127A

   Plasmid

   #140451

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA GAL4 CREB 1-341

   Plasmid

   #22972

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

   Insert

   CREB
   

   Use

   Tags

   GAL4 DNA-binding domain

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3 Flag Rab1a N124I

   Plasmid

   #46778

   Purpose

   expression of N124I Rab1a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab1a (Rab1a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N124I cannot stably bind guanine nucleotide

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-PKAsub-YPet-NES

   Plasmid

   #138203

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA pDEST40 Cas9 GW STOP

   Plasmid

   #183196

   Purpose

   Destination vector

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FRB-LplA(W37I)

    Plasmid

    #34840

    Depositor

    Alice Ting

    Article

    Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

    Insert

    FRB-LplA(W37I)
    

    Use

    Tags

    FLAG (internal fusion), HA, and His Tag

    Expression

    Mammalian

    Mutation

    LplA has Trp37 to Val mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO/Flag-mKO2

    Plasmid

    #110371

    Purpose

    Mammalian expression of mKO2 with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    monomeric Kusabira Orange 2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-GFP-LplA(AAG)

    Plasmid

    #61820

    Purpose

    Contains GFP tag for direct live-cell fluorescence imaging of the resofurin ligase

    Depositor

    Alice Ting

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

    Insert

    E. coli lipoic acid ligase
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    E20A, F147A, H149G

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA6 MCV LTco S147A

    Plasmid

    #112192

    Purpose

    Expression of MCPyV LT mutant (Ser 147 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

    Plasmid

    #157692

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3-FLAG-RIPK1 K163R

    Plasmid

    #78844

    Purpose

    To overexpress RIPK1 K163R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K163R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid

    #157699

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3-PARL-D319A-FLAG C-t

    Plasmid

    #191958

    Purpose

    Expression of Presenilin Associated Rhomboid Like (PARL) D319A mutant

    Depositor

    Luca Scorrano

    Insert

    PARL D319A
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D319A

    Promoter

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-JNKsubDD-YPet-NES

    Plasmid

    #84633

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsubDD-YPet-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-hygro-eGFP-HEC1-9D

    Plasmid

    #114029

    Purpose

    Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9D (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 9D

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-TTF-1-AD1-deletion

    Plasmid

    #75265

    Purpose

    Expresses a deletion mutant of human TTF-1 lacking the activation domain 1

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    TTF-1 (NKX2-1 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    AD1 (activation domain 1) deleted

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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