We narrowed to 18,393 results for: Tec

1. pGEX-4T-1-p19-T7-GagB500

   Plasmid

   #46314

   Depositor

   Judy Lieberman

   Article

   Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

   Inserts

   p19
   GagB500 DNA-1
   linker
   GagB500 DNA-2
   

   Use

   Pro-sirna

   Tags

   6XHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. psecBFP2

   Plasmid

   #62230

   Purpose

   encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

   Depositor

   Erik Snapp

   Article

   Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

   Insert

   secBFP2
   

   Use

   Tags

   secbfp2

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. 6his R.3-AR GUSpET28a+/pLysS

   Plasmid

   #61157

   Purpose

   N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

   Depositor

   Ichiro Matsumura, Andrew Ellington

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   GUSAR
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

   Promoter

   T7

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TAL2380

   Plasmid

   #36789

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   ABL1-TALEN_Left (Abl1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. Park2_L (TAL 3012)

   Plasmid

   #36004

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   TAL array targeting Park2 (prkn Zebrafish)
   

   Use

   T7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Park2_R (TAL 3013)

   Plasmid

   #36005

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   TAL array targeting Park2 (prkn Zebrafish)
   

   Use

   T7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBSWHhc

   Plasmid

   #133901

   Purpose

   Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCellFree_G03 YWHAB

   Plasmid

   #67063

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   YWHAB (YWHAB Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pconst #2-RFP

   Plasmid

   #81076

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Paul Adams, Jay Keasling

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter- BBa_J23104

   Available since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SEPT11 sh2 shRNA

    Plasmid

    #122023

    Purpose

    Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 shRNA (Sept11 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Zebrafish-gRNA-0006

    Plasmid

    #42246

    Purpose

    gRNA targeted to zebrafish gene rgs4

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-rgs4 (rgs4 Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGEM4Z globin NB Probe

    Plasmid

    #120390

    Purpose

    Allows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probe

    Depositor

    Niels Gehring

    Article

    Voigt et al Nat Protoc. 2019 May;14(5):1603-1633. do

    Insert

    beta globin partial 3' UTR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFPL-Yg

    Plasmid

    #61655

    Purpose

    Gateway vector for generating yellow fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGC4785

    Plasmid

    #113409

    Purpose

    Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

    Depositor

    Adam Arkin, Guillaume Cambray

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Insert

    359_31121113_2
    

    Use

    Synthetic Biology

    Tags

    fused to codon deptimized sfGFP with Flexible lin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TAL2381

    Plasmid

    #36790

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    ABL1-TALEN_Right (Abl1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. CP1300-CBEmax

    Plasmid

    #119805

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1300) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ptp4a3_L (TAL 3000)

    Plasmid

    #35992

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting ptp4a3 (ptp4a3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pATI (pKD-G7)

    Plasmid

    #62686

    Purpose

    Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    amiR-eGFP123/amiR-eGFP419
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMB1-spect-PthrC4-eGFP

    Plasmid

    #107410

    Purpose

    This plasmid contain promoter PthrC4, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PthrC4

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEos2-Talin-ABM-10

    Plasmid

    #57421

    Purpose

    Localization: Talin Actin Binding Module, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin AMB
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Actin Binding Module; I/LWEQ = 197 amino acids fr…

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits