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ND4 alphaDdCBE T2

Plasmid

#221985

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-T7-Nla-repeat-BbsI

Plasmid

#180215

Purpose

For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046979

Plasmid

#145611

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTORKm43GWF

Plasmid

#112899

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB236

Plasmid

#68627

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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PROM5_MpEF1a

Plasmid

#136113

Purpose

Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpEF1a Mapoly0024s0116

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pUBQ10-mNeonGreen-GW

Plasmid

#209352

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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p35S-GW-3Avi

Plasmid

#127918

Purpose

Gateway destination vector for transcient expression of proteins with C-terminal 3_Avi in plant protoplast

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Type

Empty backbone

Tags

3xAviTag

Expression

Plant

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB327

Plasmid

#68655

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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MTK2_007

Plasmid

#123702

Purpose

Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pEF1a

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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mNG-sfTq2ox

Plasmid

#124219

Purpose

Cytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2ox

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-sfTq2ox

Expression

Mammalian

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046920

Plasmid

#145717

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp3

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16

Plasmid

#153524

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#16

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#16

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRoLuc

Plasmid

#135284

Purpose

GBPart - RoLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

R. ohbai Luciferase

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB112

Plasmid

#68566

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB313

Plasmid

#68641

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAF259

Plasmid

#105494

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOpt

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

Bitlucopt

Use

Atc-dependent expression in c. difficile

Promoter

Ptet

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CcVA

Plasmid

#24588

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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IZ501: pMVP/Lenti-DEST

Plasmid

#121848

Purpose

pMVP destination vector, empty Lentivirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE1108

Plasmid

#202772

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCK364.306

Plasmid

#192642

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PclpB riboswitch GFP pZa

Plasmid

#170078

Purpose

GFP expression under the control of E. coli clpB promoter engineered with riboregulators

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PclpB

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-4CL1

Plasmid

#35947

Depositor

Claudia Schmidt-dannert

Article

Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

Insert

4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac-mod

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pJB38-NWMN2930

Plasmid

#84457

Purpose

pJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

Staphylococcus aureus NWMN genomic region NWMN29-30

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB302

Plasmid

#68630

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMS13: pTarget(AvCAST)

Plasmid

#168146

Purpose

Target plasmid containing AvPSP1 and attachement site for AvCAST.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PAM for AvCAST + AvPSP1
glmS

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRU53

Plasmid

#167679

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Gateway-DSCP

Plasmid

#71506

Purpose

flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB204

Plasmid

#68595

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

GUS

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWR

Plasmid

#112900

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge