We narrowed to 5,913 results for: pcDNA 3.1

1. eGFP-hCOL1A1

   Plasmid

   #140110

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag, K20…

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRB-NLuc

   Plasmid

   #31181

   Depositor

   David Piwnica-Worms

   Article

   Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

   Insert

   Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCS-13X Linker-BioID2-HA

   Plasmid

   #80899

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Klotho (secreted)

   Plasmid

   #17713

   Depositor

   Hal Dietz

   Article

   Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

   Insert

   Klotho (KL Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICS Po-Mt Short P2A

   Plasmid

   #164116

   Purpose

   Detect the short Peroxisomes-mitochondria contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPLICS Po-Mt Long P2A

   Plasmid

   #164115

   Purpose

   Detect the long Peroxisomes-mitochondria contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HaloTag-sec61b

   Plasmid

   #186959

   Purpose

   Express sec61b connected with N-terminus HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Sec61 (SEC61B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. perbB2-EGFP

   Plasmid

   #39321

   Depositor

   Martin Offterdinger

   Article

   Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

   Insert

   erbB2 (ERBB2 Human)
   

   Use

   Tags

   egfp

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0079 RESCUE-S

   Plasmid

   #130662

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-V1-ORF

    Plasmid

    #73635

    Purpose

    Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-V2-ORF

    Plasmid

    #73636

    Purpose

    Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. eGFP-MYO5B

    Plasmid

    #239265

    Purpose

    Expression of N terminal GFP tagged Myo5B

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    MYO5B (MYO5B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Marisa Brini, Tito Calì

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HDAC5 Flag

    Plasmid

    #13822

    Purpose

    Mammalian expression of human histone deacetylase 5 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIRT1 Flag

    Plasmid

    #13812

    Purpose

    Mammalian expression of human SIRT1 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    N-terminus truncated

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0096 RESCUE truncation (Cas13 delta 892-1095)

    Plasmid

    #130663

    Purpose

    CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b(delta 892-1095)-ADAR2dd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEV53D

    Plasmid

    #44168

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Insert

    Equine infectious anemia virus (EIAV) gag-pol,rev, and S2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A131T mutation in gag; Deletion of viral LTR sequ…

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. deltaNp63alpha-FLAG

    Plasmid

    #26979

    Depositor

    David Sidransky

    Article

    Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

    Insert

    ΔNP63alpha (TP63 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    dominant negative isoform of p63; aa 1–587

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC0078 RESCUE

    Plasmid

    #130661

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd (RESCUE)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Mfn1-Myc

    Plasmid

    #23212

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn1 (Mfn1 Mouse)
    

    Use

    Tags

    6xHis, 9xMyc, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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