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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,259 results for: pcDNA 3.1

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Showing: 4821 - 4840 of 6259 results

  1. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid
    #70229
    Purpose
    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Tags
    3XFLAG, FK506- and rapamycin-binding protein (FKB…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. mito-mGarnet

    Plasmid
    #104309
    Purpose
    codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito
    Depositor
    Ulrich Nienhaus
    Article
    Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103
    Insert
    mito-mGarnet
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104309-plasmid-map-sequence-id-225634

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  8. mVenus-hCOL1A1

    Plasmid
    #140111
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140111-plasmid-map-sequence-id-296751

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  15. ER2-LSSmGFP

    Plasmid
    #186538
    Purpose
    Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available Since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186538-plasmid-map-sequence-id-368236

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Showing: 4821 - 4840 of 6259 results