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ePB-PURO-FLAG-METTL3

Plasmid

#160252

Purpose

For stable integration of FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Syn:eGFP-CaM-uTEV1Δ(220-242)

Plasmid

#135463

Purpose

Neuronal expression of uFLARE protease component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

eGFP-CaM-uTEV1Δ

Use

AAV

Tags

eGFP, V5

Expression

Mammalian

Mutation

S219V mutation improves stability and S153N impro…

Promoter

Synapsin

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:ChR2(H134R)-tdTomato

Plasmid

#124237

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChR2(H134R)-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMP4518

Plasmid

#107778

Purpose

Expression of EYFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pBBR1-mcs5

Tags

pLacZ-EYFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Actin-7

Plasmid

#58001

Purpose

Localization: Actin, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Actin (ACTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
SmoM2 (W535L)-pcw107

Plasmid

#64627

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

SMO (SMO Human)

Use

Lentiviral

Mutation

W535L

Promoter

PGK

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only
BCL-XL-pcw107-V5

Plasmid

#64631

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

BCL2L1 (transcript variant 1) (BCL2L1 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3/FLHIP1

Plasmid

#29587

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pIS1-miR-206 site

Plasmid

#25027

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-206 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
995 pSG5L HA PTEN S370A

Plasmid

#10754

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

S370A

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
997 pSG5L HA PTEN T382A

Plasmid

#10757

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

T382A

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pDN-D2irG4kwh

Plasmid

#44723

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
EGFP

Use

Synthetic Biology; Expression regulator/reporter

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-Wt-PTEN

Plasmid

#206982

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTEN

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tet-on promoter

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-FRA1

Plasmid

#188665

Purpose

Expresses murine FRA1 (Fosl1)

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCB301TOR-CRISPR

Plasmid

#164797

Purpose

For gene editing in oomycetes

Depositor

Miaoying Tian

Article

Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

Inserts

PsNLS-hSpCas9
sgRNA expression cassette

Use

Oomycete expression

Promoter

HAM34 and RPL41

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO2-TVA-mCherry

Plasmid

#149297

Purpose

Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

TVA:mCherry

Use

AAV

Mutation

Start codon removed.

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-D169G

Plasmid

#133757

Purpose

Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed aspartate 169 to glycine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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ETV4_pet28a

Plasmid

#131645

Purpose

Bacterial expression of ETS gene ETV4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV4 (ETV4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127315

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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