We narrowed to 12,459 results for: 109

1. mcry1K31R HA tag

   Plasmid

   #110297

   Purpose

   mammalian expression of full length mouse CRY1 mutant K31R

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   mcry1 K31R (Cry1 Mouse)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   31 Lysines are changed to Argenine

   Promoter

   CMV

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-Blast POLD1

   Plasmid

   #160805

   Purpose

   Express POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized C-terminal 230 amino acids

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. phBG-P53DD

   Plasmid

   #149707

   Purpose

   In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

   Depositor

   Erika Sasaki

   Article

   Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Rna transcription

   Tags

   Expression

   Mutation

   deleted amino acid 14-301

   Promoter

   T7

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N3-CYB5R3

   Plasmid

   #204567

   Purpose

   Expresses EGFP-tagged CYB5R3 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CYB5R3 (CYB5R3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-RasAR(R89L)-NES

   Plasmid

   #205568

   Purpose

   RasAR negative-control mutant plasmid. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR(R89L)-NES (RAF1 Synthetic, Human)
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Arg 89 mutated to Leu in Raf1 RBD region.

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdEos-Mito-7

   Plasmid

   #57644

   Purpose

   Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCold I-Hero20

   Plasmid

   #187930

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero20

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero20 (C11orf58 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CDKL1_HUMAN_D0

   Plasmid

   #79699

   Purpose

   This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CDKL1 (CDKL1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. MBP-hnRNPA2_deltaLC

   Plasmid

   #98664

   Purpose

   Expresses MBP-tagged hnRNPA2 without the LC domain

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   G190Stop

   Promoter

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86020

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 82 to valine, threonine 230 to al…

    Promoter

    CMV IE

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-AR-R405A

    Plasmid

    #126052

    Purpose

    Expresses human androgen receptor (R405A) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R405A

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-NSD3-short W284A

    Plasmid

    #72551

    Purpose

    expresses 3*FLAG tagged human NSD3-short with W284A mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    W284A

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PHR-GFP-FUSN-VP16

    Plasmid

    #183933

    Purpose

    Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-FUSN-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307-Bat-CoV-HKU9-3CL-C144A

    Plasmid

    #160285

    Purpose

    Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C144A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV10-Flag-Ovtm

    Plasmid

    #51888

    Purpose

    expression vectore in mammalian cells

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    mFbxl3 (Fbxl3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ovtm (I364T)

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

16. MBP-hnRNPA2_FL_D290V

    Plasmid

    #98663

    Purpose

    Expresses MBP-tagged full length hnRNPA2 with D290V

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0584

    Plasmid

    #142651

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Desmoplakin II tension sensor (FL-based)

    Plasmid

    #118721

    Purpose

    The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-FL-mCherry

    Expression

    Mammalian

    Mutation

    inserted FL-based tension sensor module after aa1…

    Promoter

    CMV

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MBP-hnRNPA2_FL_P298L

    Plasmid

    #104468

    Purpose

    express MBP-tagged full length hnRNPA2 P298L

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    P298L

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3xFLAG CMV14 / BRAF-D594V

    Plasmid

    #131715

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D594V

    Promoter

    CMV

    Available since

    May 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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