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pCAG-TWIN-STREP-FLAG-NPRL3

Plasmid

#164984

Purpose

NPRL3 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL3 (NPRL3 Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep78

Plasmid

#136827

Purpose

Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep78 (CEP78 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

Plasmid

#127357

Purpose

gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts1

Plasmid

#115862

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-C9ORF80

Plasmid

#128418

Purpose

Express C9ORF80 in E. coli with a His tag (C-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

C9ORF80 (INIP Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-ATF5-201

Plasmid

#117340

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR ATF5-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Murine PGK-1

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV Puro SCRIB P305L

Plasmid

#88887

Purpose

SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Mutation

Proline to Leucine mutation @ 305th amino acid

Promoter

MSCV promoter

Available Since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMyc-C1-Cep123 (CCDC123/Cep89)

Plasmid

#69836

Purpose

This plasmid encodes Cep123 (CCDC123/Cep89) isoform 1 with an N-ter Myc-tag

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAW-EGFP-4xlet-7 (pAW-AS27)

Plasmid

#40834

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Four target sites perfectly complementary to let-7

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pEB1deltaC-2xEGFP shRNA resistant

Plasmid

#37925

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion after aa248, TTCCTGT=>TCCCGGT

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate1-N1

Plasmid

#31870

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-deltaNLS1-EGFP

Plasmid

#22150

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 297-304

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB7

Plasmid

#19503

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB7 (DNAJB7 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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HOXA4 (murine) HA-tag pET

Plasmid

#8489

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXA4 (Hoxa4 Mouse)

Tags

HA and T7

Expression

Bacterial

Mutation

Thr-2 changed to Ala-2 to form better ribosomal b…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP_D2A_tdTomato

Plasmid

#184045

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 LRP6-APEX2-Ires-eGFP-PAC

Plasmid

#180142

Purpose

used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Expression

Mammalian

Promoter

SP6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRepair-mScI-CTNNB1

Plasmid

#153431

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mScarlet-i

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4580NES

Plasmid

#138376

Purpose

Booster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-JNK

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

Plasmid

#99679

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VP64-p65(101-261)-RTA(125-190)

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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