We narrowed to 26,734 results for: STI

1. JT504: pMVP/SB/eGFP-DEST

   Plasmid

   #121861

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. JT601: pMVP/SB/mCherry-DEST

   Plasmid

   #121862

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPQD1CB0107

   Plasmid

   #177040

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF11-SFPQ(214-598)

   Plasmid

   #135440

   Purpose

   Expresses SFPQ 214-598 with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. VRER variant

   Plasmid

   #138561

   Purpose

   Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRER
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPQD1CB0202

   Plasmid

   #185626

   Purpose

   MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 merlin 308-590

   Plasmid

   #11627

   Depositor

   Vijaya Ramesh

   Article

   Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

   Insert

   merlin (NF2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Carboxy-terminal half of merlin isoform 2.

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

8. px335-sgRNA-EF(Cas9n)-Eif4a1-R

   Plasmid

   #122345

   Purpose

   Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Eif4a1
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF11-SFPQ(276-535)

   Plasmid

   #135436

   Purpose

   Expresses SFPQ 276-535 with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

    Plasmid

    #110831

    Purpose

    Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.

    Depositor

    Ande Satyanarayana

    Article

    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

    Insert

    Azgp1 (Azgp1 Mouse)
    

    Use

    Non-viral gene expression vector

    Tags

    dTomato

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs_FLAG-FSF1

    Plasmid

    #110641

    Purpose

    Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    FSF1 (FSF1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-Climp63(1–301)

    Plasmid

    #186956

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Climp63(1–437)

    Plasmid

    #186958

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAA3

    Plasmid

    #154163

    Purpose

    Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Mutation

    20 wt NF-κB sites were replaced with Rel1A-specif…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid

    #80272

    Purpose

    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET19b-hMED9

    Plasmid

    #15365

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

    Insert

    hMED9 (MED9 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. 360 pCR kdrb

    Plasmid

    #13067

    Depositor

    Nathan Lawson

    Article

    Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

    Insert

    kdrb (kdr Zebrafish)
    

    Available Since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET28-At_PRORP1-His

    Plasmid

    #67869

    Purpose

    bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP1 (PRORP1 Mustard Weed)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 36-572 (see comments)

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 GATA1 K137R

    Plasmid

    #85694

    Purpose

    Mammalian expression of mouse GATA1 with K137R mutation

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Expression

    Mammalian

    Mutation

    K137R (SUMO-1 attachment site)

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pEGFP-CD86-mApple

    Plasmid

    #133860

    Purpose

    Mammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    CD86 (CD86 Human)
    

    Tags

    EGFP and mApple

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHelA12

    Plasmid

    #176493

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

    Depositor

    Jian-Dong Huang, Xiaodong Cheng

    Article

    Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

    Insert

    SARS-CoV NSP13 (ORF1ab SARS-CoV)
    

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    V570I

    Promoter

    T7

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

    Plasmid

    #37606

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP III C840A (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C840A

    Promoter

    CMV tet regulatable

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pPN461

    Plasmid

    #137877

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

    Plasmid

    #101202

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant M1–N497,GGS,V713–D1368
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    M1–N497, GGS, V713–D1368

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEMS2131

    Plasmid

    #111895

    Purpose

    ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.2/NS5-Af-mut

    Plasmid

    #120829

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    ZIKV NS5 V114M/V872M - "african mut"
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    V114M/V872M

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KN302: pMVP/SB/eCFP-DEST

    Plasmid

    #121863

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KK901: pMVP/SB/Zeo-DEST

    Plasmid

    #121868

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. R4pGWB401+Citrine

    Plasmid

    #128432

    Purpose

    Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Citrine, yellow fluorescence protein

    Expression

    Plant

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEMS1964

    Plasmid

    #82574

    Purpose

    High copy number plasmid containing Ple305 MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple305
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 23, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pLentiCRISPR sgHAL

    Plasmid

    #102315

    Purpose

    genetic depletion of HAL

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgHAL (HAL Human)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. px330 CTCF gRNA

    Plasmid

    #165588

    Purpose

    Insertion of CTCF degon

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    CTCF (CTCF Mouse)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. px330 CBP gRNA

    Plasmid

    #165587

    Purpose

    insertin of CBP degrom

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    CBP (Crebbp Mouse)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPN460

    Plasmid

    #137878

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHL 1917

    Plasmid

    #53024

    Purpose

    AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    mCherry
    fimB (fimB E. coli)
    

    Use

    Synthetic Biology

    Tags

    Asp terminator, RBS(st7), and T1 terminator

    Expression

    Bacterial

    Promoter

    pCon and pLlacO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA5 HA-CCDC95

    Plasmid

    #19795

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    CCDC95 (INO80E Human)
    

    Tags

    2XHA

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

39. pTV-mHdac1-tdiRFP670-PEST2

    Plasmid

    #122351

    Purpose

    Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    2A-tdiRFP-PEST with homology arms for Hdac1
    

    Use

    Mouse Targeting

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX-4T1-Claspin-BPII

    Plasmid

    #31632

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 492-622 of claspin

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only