We narrowed to 11,716 results for: VARS
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Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBad-PhoCl2f
Plasmid#164036PurposeImproved PhoCl variant with faster dissociation rateDepositorInsertPhoCl2f
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6s
Plasmid#105714PurposeCan be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.DepositorHas ServiceAAV8InsertGCaMP6s
UseAAV; Flp/frtExpressionMammalianPromoterEf1a, Human elongation factor-1 alphaAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH-tdGFP
Plasmid#74322PurposepH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiaeDepositorInsertpH-tdGFP
UseYeast taggingTagsnoneExpressionYeastMutationN149Y, Q204HAvailable SinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
LT3GEP
Plasmid#111172PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorTypeEmpty backboneUseLentiviralTagsEGFPPromoterT3GAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-Int-SpyTag
Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
TagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS:iGluSnfr.zf1
Plasmid#108356PurposeUAS driven variant of iGluSnFR for zebrafish expressionDepositorInsertUAS:[GlyRa1 secretion sequence]-[iGluSnFR]
UseZebrafish expressionPromoterlacAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_CMV_MeltCasp1-37-GFP
Plasmid#234069PurposeEncodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°CDepositorInsertMeltCasp1-37-GFP
UseLentiviralExpressionMammalianMutationMelt(C292A,Q355N); hcaspase1(R391K)PromoterCMVAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
LT3REV
Plasmid#111174PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorTypeEmpty backboneUseLentiviralTagsDsRed and VenusPromoterT3GAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR hH1-TERRA
Plasmid#111381PurposeTERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminatorDepositorInserthH1-TERRA
UseGateway entry cloningPromoterhH1Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _2
Plasmid#78202PurposeExpression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A D63C T174A T175N D224C C287A)
TagsHis6ExpressionBacterialMutationK33A D63C T174A T175N D224C C287APromoterunknownAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-Rps7-meGFP
Plasmid#241318PurposeMouse Rps7 fused to meGFP for expression in mammalian cellsDepositorAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
RT3REVIR
Plasmid#111168PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorTypeEmpty backboneUseRetroviralTagsdsRed and mVenusPromoterT3GAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFD2-dU6:2gRNA
Plasmid#49409Purposeexpression of gRNA under control of the Drosophila U6:2 promoterDepositorInsertdU6-2:gRNA
UseCRISPRExpressionInsectPromoterdU6-2Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-CitronRH
Plasmid#158545PurposeMammalian expression of nonbinding variant of direct-response citrate sensorDepositorInsertCitronRH
ExpressionMammalianPromotercmvAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p458 VQR
Plasmid#101727PurposeExpresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifsDepositorInsertSpCas9 VQR
ExpressionMammalianMutationVQRAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE2_CMV_Mex3a_t2a_mCherry
Plasmid#223244PurposetetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherryDepositorInsertMex3a (Mex3a Mouse)
TagsmCherryExpressionMammalianMutationamino acids conserved, underlying DNA sequence re…PromoterCMVAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-ND1 Left -GSVG-UGI
Plasmid#187412Purposehuman mitochondrial DNA ND1 target TALEDepositorInsertDddA GSVG variant
ExpressionMammalianAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-ERBB2-N-18
Plasmid#62755PurposeLocalization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509DepositorAvailable SinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ii-Str_neomycin
Plasmid#65308PurposeLuminal ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
ExpressionMammalianMutationStreptavidin in its lumenal partPromoterCMVAvailable SinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-H-Lock
Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
eNme2C-CBE6c
Plasmid#215831PurposeExpress CBE6c (with eNme2-C variant) in mammalian cellsDepositorInsertCBE6c-eNme2C-2xUGI
ExpressionMammalianAvailable SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _1
Plasmid#78201PurposeExpression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A I27C T174A T175N C287A)
TagsHis6ExpressionBacterialMutationK33A I27C T174A T175N C287APromoterunknownAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL0_20 [panARS-opt ORI]
Plasmid#198933PurposeLevel 0 partDepositorInsertpanARS-opt ORI
UseSynthetic BiologyAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_puromycin
Plasmid#65309PurposeLuminal ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
ExpressionMammalianMutationStreptavidin in its lumenal partPromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+18)
Plasmid#199162Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+18)
TagsMGHHHHHHGGAMutationE16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+12)
Plasmid#199161Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+12)
TagsMGHHHHHHGGAMutationE16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-LRF
Plasmid#66936PurposeDestination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).DepositorTypeEmpty backboneUseLuciferaseExpressionMammalianPromoterSV-40Available SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-CitroffRH
Plasmid#158546PurposeMammalian expression of nonbinding variant of inverse-response citrate sensorDepositorInsertCitroffRH
ExpressionMammalianPromotercmvAvailable SinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
ExpressionBacterialMutationSUMO tag fused to N-terminus of PylBAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2_166
Plasmid#199098PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertRK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH643
Plasmid#172168PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPara sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
ExpressionBacterialMutationsfGFP(N39*, N135*, Y151*)Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2_167
Plasmid#199099PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB007ns2a
Plasmid#99214PurposeAccessory plasmid for PACE of PylRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
Mutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PtetAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only