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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,191 results for: his

Showing: 9721 - 9760 of 22191 results

  7. pBAD-MTX-u36_Snifit

    Plasmid
    #119045
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u36_Snifit
    Use
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    36TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119045-plasmid-map-sequence-id-229505

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  8. pBAD-MTX-u51_Snifit

    Plasmid
    #119046
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u51_Snifit
    Use
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    51TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119046-plasmid-map-sequence-id-229503

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  9. pBAD-MTX-u23_Snifit

    Plasmid
    #119044
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u23_Snifit
    Use
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    23TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119044-plasmid-map-sequence-id-229496

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  15. Halo-Tev-Akt3 C119S-2X Flag

    Plasmid
    #87608
    Purpose
    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of C119S mutant of human Akt3 protein
    Depositor
    Yimon Aye
    Insert
    AKT3 isoform 2 (AKT3 Human)
    Use
    Zebrafish (danio rerio) expression
    Tags
    2 tandem flag tags and His-Halo-Tev
    Expression
    Mammalian
    Mutation
    cysteine 119 residue mutated to serine
    Promoter
    Available Since
    Nov. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87608-plasmid-map-sequence-id-197921

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  28. pfMDH_Triple

    Plasmid
    #67733
    Purpose
    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine, arginine 373 to…
    Promoter
    T7
    Available Since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. pAD-g6

    Plasmid
    #61497
    Purpose
    For expression of C. briggsae gld-1, used for rescue of C. elegans q485 homozygotes
    Depositor
    Eric Haag
    Article
    Beadell et al Proc Natl Acad Sci U S A. 2011 Dec 6;108
    Insert
    gld-1 (Cbr-gld-1 Caenorhabditis briggsae)
    Use
    Tags
    FLAG and His
    Expression
    Worm
    Mutation
    Promoter
    gld1
    Available Since
    Feb. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. pET-Ce25

    Plasmid
    #51296
    Purpose
    Expresses a plant arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7
    Insert
    arginyl-tRNA synthetase
    Use
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    GTT (Val) insertion after initiation codon to per…
    Promoter
    T7
    Available Since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    51296-plasmid-map-sequence-id-78029

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Showing: 9721 - 9760 of 22191 results