We narrowed to 19,024 results for: Gateway

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R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

Plasmid

#159579

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:GFPNLS-SV40pA

Plasmid

#155123

Purpose

Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-hsp70I:QFGal4-SV40pA

Plasmid

#155120

Purpose

Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

hsp70l:QFGal4 (hsp70l Zebrafish, Budding Yeast, Neurospora crassa)

Use

Zebrafish expression

Promoter

hsp70l

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_BirA

Plasmid

#136537

Purpose

Lentiviral expression vector for an inducible BirA

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_BirA_NRF2

Plasmid

#136539

Purpose

Lentiviral expression vector for an inducible BirA-NRF2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA-NRF2 (NFE2L2 Human)

Use

Lentiviral; Destination vector for gateway cloning

Expression

Mammalian

Available Since

June 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-K298M

Plasmid

#140314

Purpose

Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-ALMS1

Plasmid

#136828

Purpose

Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-ALMS1 (ALMS1 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4591NES

Plasmid

#138377

Purpose

Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-ROCK

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:icre-p2a-tomato

Plasmid

#135217

Purpose

Cre coexpressed with tdTomato in macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter with iCre and Tomato

Use

Cre/Lox; Zebrafish expression

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pME tdTomato

Plasmid

#135202

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

tdTomato

Use

Synthetic Biology

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG

Plasmid

#116774

Purpose

Lentiviral expression of PIK3CG

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

WT

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA, mitfa:Cas9

Plasmid

#118840

Purpose

Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-WDR12

Plasmid

#116803

Purpose

Lentiviral expression of WDR12

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

WDR12 (WDR12 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SMAD4

Plasmid

#116791

Purpose

Lentiviral expression of SMAD4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP3K4

Plasmid

#116759

Purpose

Lentiviral expression of MAP3K4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP3K4 (MAP3K4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT4

Plasmid

#116745

Purpose

Lentiviral expression of FLT4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT4 (FLT4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BMPR2

Plasmid

#116718

Purpose

Lentiviral expression of BMPR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BMPR2 (BMPR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CD-E1021K

Plasmid

#116566

Purpose

Lentiviral expression of PIK3CD E1021K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CD (PIK3CD Human)

Use

Lentiviral

Mutation

E1021K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP2K1-K57N

Plasmid

#116427

Purpose

Lentiviral expression of MAP2K1 K57N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

K57N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-HLA-A0201-His

Plasmid

#108213

Purpose

HLA-A0201 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-A Human)

Use

Entry vector for gateway system

Tags

his

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:KIT L576P

Plasmid

#118848

Purpose

Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

KIT L576P (KIT Human, Zebrafish)

Use

Zebrafish expression, tol4

Mutation

KIT L576P

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-CELF4

Plasmid

#115445

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

CELF4 (CELF4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Non-specific sgRNA

Plasmid

#109432

Purpose

MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Non-specific gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_AIFM1

Plasmid

#111659

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AIFM1 (AIFM1 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-px330-CRBN-T2-pGK-Pur

Plasmid

#107383

Purpose

Mammalian expression CRISPR/Cas9

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

sgRNA targeting CRBN (CRBN Human)

Use

Lentiviral; Crispr

Expression

Mammalian

Promoter

U6

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast-CDT1-HA

Plasmid

#109335

Purpose

virus packaged, doxycyline inducible expression of Cdt1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cdt1 (CDT1 Human)

Use

Lentiviral; Inducible expression

Tags

HA, His, Strep

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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OLIG2_pCSdest

Plasmid

#53858

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

OLIG2 (OLIG2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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TTC3_pCSdest

Plasmid

#53851

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TTC3 (TTC3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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IL10RB_pCSdest

Plasmid

#53799

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IL10RB (IL10RB Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
ADAMTS1_pCSdest

Plasmid

#53807

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ADAMTS1 (ADAMTS1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pLenti-Tet-MKK6(DD)-Puro

Plasmid

#86094

Purpose

Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

Depositor

Cindy Miranti

Article

Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

Insert

MKK6 (MAP2K6 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Serine 207 and Threonine 211 both changed to Aspa…

Promoter

CMV/TetO

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

Plasmid

#69049

Purpose

lentiviral expression of IKZF2 variant 2 with FLAG tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2 (IKZF2 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

splice variant 2

Promoter

CAG

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP

Plasmid

#69048

Purpose

lentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

splice variant 1, Q146H

Promoter

CAG

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_SRF

Plasmid

#72695

Purpose

Doxycyline regulated expression of SRF in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

serum response factor (SRF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK TT 3xFLAG ID2 hygro

Plasmid

#98394

Purpose

Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGem-nHyPer2:sHyper2

Plasmid

#84737

Purpose

Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUB-sSypHer (RbcS-SypHer)

Plasmid

#84732

Purpose

Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

sSypHer

Tags

RbcS targeting sequence

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TAC-OK+9MS

Plasmid

#80482

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [1-483]

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Tal1

Plasmid

#60670

Purpose

PiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Tal1 (Tal1 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Sox17

Plasmid

#60669

Purpose

PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Sox17 (Sox17 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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