We narrowed to 22,833 results for: Sis

1. pBN261

   Plasmid

   #167966

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y713E, Y715E, Y717E

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBN081

   Plasmid

   #173443

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y705E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y705E
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y705E

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBN082

   Plasmid

   #173444

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y706E
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y706E

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBN083

   Plasmid

   #173445

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y705E, Y706E

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBN084

   Plasmid

   #173446

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y708E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y708E
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y708E

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBN085

   Plasmid

   #173447

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y713E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y713E
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y713E

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBN008

   Plasmid

   #167949

   Purpose

   express E. coli E69 (O9a:K30) Wzc with K540M mutation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBN093

   Plasmid

   #167951

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, delete motif 3

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBN074

   Plasmid

   #167955

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y705E, Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y705E, Y706E
   

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y705E, Y706E

   Promoter

   araBAD

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TetO::EGFP-hMeikin(8A mut)

    Plasmid

    #174707

    Purpose

    Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBHRSF228

    Plasmid

    #135007

    Purpose

    MusF2 prenyltransferases from Nostoc sp. UHCC 0398

    Depositor

    Hideo Iwai

    Article

    Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

    Insert

    MusF2 prenyltransferase
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS426-CeLACE-1

    Plasmid

    #174523

    Purpose

    Express C.elegans LACE-1 in yeast cells

    Depositor

    Oleh Khalimonchuk

    Article

    Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

    Insert

    lace-1 (C30F12.2 Nematode)
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJL15

    Plasmid

    #174069

    Purpose

    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    pGal1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

    Plasmid

    #173462

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

    Plasmid

    #173460

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS41K-mCherry-mNeonGreen (pJJF003)

    Plasmid

    #173459

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

    Plasmid

    #173458

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pThuAsol

    Plasmid

    #171675

    Purpose

    pThuAsol expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    SUMO-TEV-ThuAsol
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC-U63-tgRNA-Gal80

    Plasmid

    #169030

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-ABEmax(TadA E59Q, TadA* V106W)

    Plasmid

    #126496

    Purpose

    Expresses ABEmax(TadA E59Q, TadA*V106W) in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

    Insert

    ABEmax(TadA E59Q TadA* V106W)
    

    Expression

    Mammalian

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-ABEmax(TadA E59, TadA* V106W)

    Plasmid

    #126497

    Purpose

    Expresses ABEmax(TadA E59, TadA*V106W) in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

    Insert

    ABEmax(TadA E59 TadA* V106W)
    

    Expression

    Mammalian

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AP-VAMP7(low)

    Plasmid

    #167998

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP7(K121-R180)-T7PAP
    

    Use

    Synthetic Biology

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-CV-VAMP1

    Plasmid

    #168022

    Purpose

    Expresses eCFP-VAMP1(T29-K89)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP1(T29-K89)-mVenus
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-CV-VAMP4

    Plasmid

    #168025

    Purpose

    Expresses eCFP-VAMP4(P48-R107)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP4(P48-R107)-mVenus
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-CV-VAMP3

    Plasmid

    #168024

    Purpose

    Expresses eCFP-VAMP3(S11-R69)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP3(S11-R69)-mVenus
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-CV-VAMP7

    Plasmid

    #168027

    Purpose

    Expresses eCFP-VAMP7(K121-R180)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP7(K121-R180)-mVenus
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-CV-VAMP5

    Plasmid

    #168026

    Purpose

    Expresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP5(M1-Q60)-mVenus
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWL89A mmPing20F

    Plasmid

    #145789

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20F
    

    Expression

    Yeast

    Mutation

    Insertion of the FMV enhancer

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWL89A mmPing20B

    Plasmid

    #145790

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20B
    

    Expression

    Yeast

    Mutation

    Insertion of the b-Conglycin Enhancer

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWL89A NL60

    Plasmid

    #145791

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    NL60
    

    Expression

    Yeast

    Mutation

    Insertion of the OCS Enhancer

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pWL89A 2xE

    Plasmid

    #145792

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    2xE
    

    Expression

    Yeast

    Mutation

    Insertion of 2 copies of the CMV Enhancer

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pWL89A 4xE

    Plasmid

    #145793

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    4XE
    

    Expression

    Yeast

    Mutation

    Insertion of 4 copies of the CMV Enhancer

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPB-tetpA(R1R4)-iRPT

    Plasmid

    #154882

    Purpose

    Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw

    Plasmid

    #162479

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreBC
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw

    Plasmid

    #162474

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreBC
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HJP-473-DNE>FLP-T2A-CreAB-NrdJ-1N

    Plasmid

    #162480

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    DNE-Frterminator
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMAL-C2X -MTalpha-FliG

    Plasmid

    #154361

    Purpose

    Bacterial expression of Mtalpha

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Mtalpha
    

    Expression

    Bacterial

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMAL-C2X -MTn-FliG

    Plasmid

    #154360

    Purpose

    Bacterial expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Expression

    Bacterial

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. as-mOPN-PB0

    Plasmid

    #106450

    Purpose

    Bacterial expression of antisense to murine osteopontin

    Depositor

    Georg Weber

    Article

    Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

    Insert

    antisense-osteopontin (Spp1 Mouse)
    

    Use

    Retroviral

    Mutation

    antisense

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge