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We narrowed to 5,485 results for: crispr cas9 grna plasmid

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Showing: 4881 - 4900 of 5485 results

  3. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80439-plasmid-map-sequence-id-151455

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  4. pSc1-puro

    Plasmid
    #80438
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV, U6, and hPGK
    Available Since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80438-plasmid-map-sequence-id-151453

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  5. pJW1328

    Plasmid
    #69488
    Purpose
    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans
    Depositor
    Jordan Ward
    Insert
    sgRNA(F+E) targeting pha-1
    Expression
    Worm
    Promoter
    R07E5.16 U6 promoter
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69488-plasmid-map-sequence-id-125217

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  7. pRARB.1.0-gDNA

    Plasmid
    #132471
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RARB (RARB Human)
    Use
    CRISPR
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132471-plasmid-map-sequence-id-267178

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  8. pJAT31

    Plasmid
    #204291
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204291-plasmid-map-sequence-id-405841

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  9. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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  10. HCP3

    Plasmid
    #166105
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166105-plasmid-map-sequence-id-330319

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  11. HCP7

    Plasmid
    #166109
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166109-plasmid-map-sequence-id-330317

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  12. pRDA_837

    Plasmid
    #245328
    Purpose
    Cas9 CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245328-plasmid-map-sequence-id-488040

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  13. pCG-ACTB-C4

    Plasmid
    #229846
    Purpose
    Expresses wild-type Cas9 and gRNA for ACTB gene.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA for ACTB gene
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229846-plasmid-map-sequence-id-457514

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  14. pCG-NT2

    Plasmid
    #229848
    Purpose
    Expresses wild-type Cas9 and gRNA with non-target sequence.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA with non-target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229848-plasmid-map-sequence-id-457513

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  16. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65779-plasmid-map-sequence-id-112660

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  17. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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  18. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  19. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

    Plasmid
    #115487
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    115487-plasmid-map-sequence-id-240326

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  20. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid
    #115490
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    115490-plasmid-map-sequence-id-238520

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Showing: 4881 - 4900 of 5485 results