We narrowed to 17,204 results for: puro

1. SpCas9

   Plasmid

   #188489

   Purpose

   Expresses FLAG tagged SpCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EK0007-PB-4XnrUAS-H2B-BFP

   Plasmid

   #236121

   Purpose

   Fuorescent reporter encoding H2B-tagBFP fusion under control of E1b minimal promoter with four Gal4-binding sites upstream

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   E1b minimal promoter with four Gal4-binding sites…

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PER2:Luc Plasmid

   Plasmid

   #206875

   Purpose

   Per2 promoter luciferase reporter

   Depositor

   Michelle Farkas

   Article

   Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

   Insert

   Period 2 (Per2 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYC-C

   Plasmid

   #207114

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW empty vector

   Plasmid

   #184708

   Purpose

   Lentiviral empty vector for doxycycline-inducible expression

   Depositor

   Alessia Ciarrocchi, Gloria Manzotti

   Article

   Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEFIRES-P-mCherry-HPos

   Plasmid

   #87159

   Purpose

   Fluorescent marker of lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   HPos
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1-alpha

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. EcSTH-LbNOX

   Plasmid

   #211924

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   EcSTH-LbNOX
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPCX mito roGFP2-Orp1

   Plasmid

   #64992

   Purpose

   Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYC-N

   Plasmid

   #207113

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX_HA

    Plasmid

    #178534

    Purpose

    Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPU-huTRIMmiR30-TRIM5

    Plasmid

    #132938

    Purpose

    All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    human TRIM5
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi_RP_MicroID2-HA

    Plasmid

    #188521

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGS403_scFv-GCN4-sfGFP-GB1-HRV3C-CENP-T[1-242 T11A, T85A] in AAVS donor

    Plasmid

    #211888

    Purpose

    CENP-T[1-242] transgene integration at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    CENP-T[1-242] (CENPT Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    T11A, T85A

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAPM-D4- miR30-L1221

    Plasmid

    #115846

    Purpose

    Control shRNA KD vector

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    control shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLIX403-hNOS2

    Plasmid

    #110800

    Purpose

    tet-inducible NOS2 expression

    Depositor

    Edward Morgan

    Article

    Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

    Insert

    NOS2 (NOS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMA3287

    Plasmid

    #46883

    Purpose

    Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

    Depositor

    Mikhail Alexeyev

    Article

    Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

    Insert

    Uracil-N-glycosylase, Y147A mutant (UNG Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Y147A

    Promoter

    TRE-Tight

    Available since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPC-NFLAG TRF1

    Plasmid

    #16058

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    aa2-439

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pPB-cT3G-cERP2-YTHDC2

    Plasmid

    #222558

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    YTHDC2 (YTHDC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV-TRE-WT human MyoD-T2A-dsRedExpress2

    Plasmid

    #60628

    Purpose

    Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    VP64 mouse MyoD (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEXPqcxip-hSURF4-FLAG

    Plasmid

    #159139

    Purpose

    Hu SURF4 -flag mammalian expression Gateway vector.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    surfeit 4 (SURF4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (cytomegalovirus) enhancer and the MSV (mouse…

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCW57-RFP-P2A-MCS

    Plasmid

    #78933

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

    Plasmid

    #121944

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEM705 Gsi (Ser)

    Plasmid

    #109352

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cys at position 391 to Serine (Pertussis …

    Promoter

    CAG

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228362

    Purpose

    mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p667-UBC-CDK12-V5_IDG-K

    Plasmid

    #135245

    Purpose

    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK12-V5 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sgLenti

    Plasmid

    #105996

    Purpose

    single sgRNA expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; S.pyogenes sgrna expressio…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMRXIP Turquoise2-Syntaxin17

    Plasmid

    #89938

    Purpose

    Expresses Syntaxin17 tagged with Turquoise2 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

    Insert

    human Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPCX-NICD

    Plasmid

    #44471

    Depositor

    Ernesto Canalis

    Article

    Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

    Insert

    Notch intracellular domain (Notch1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains amino acid 1753 to C-Term

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pZLCv2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106357

    Purpose

    Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLJC5-LAMP1-RFP-2xFlag

    Plasmid

    #102931

    Purpose

    Express a lysosomal Tag

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    mRFP-2xFlag

    Expression

    Mutation

    Promoter

    ubc

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p6659 MSCV-IP N FlagHA HPV16E6

    Plasmid

    #44152

    Depositor

    Peter Howley

    Article

    White et al J Virol. 2012 Dec;86(24):13174-86. doi:

    Insert

    HPV16E6
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

34. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLPC-Flag-PML-IV

    Plasmid

    #62804

    Purpose

    expresses the isoform IV of PML (promyelocytic leukemia protein)

    Depositor

    Gerardo Ferbeyre

    Article

    Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

    Insert

    PML-IV (PML Human)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

36. Flag pLJM1 RagB 54L

    Plasmid

    #19314

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding B (RRAGB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T54L

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CRISPR-SP-Cas9 reporter

    Plasmid

    #62733

    Purpose

    Mammalian CRISPR-SP-Cas9 reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
    

    Use

    CRISPR and Lentiviral

    Tags

    CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMM289

    Plasmid

    #46037

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN E84A/K577M (WRN Human)
    

    Use

    Tre, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A/K577M exonuclease and helicase-dead mutant; …

    Promoter

    CMV2

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMM287

    Plasmid

    #46035

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN K577M (WRN Human)
    

    Use

    Teton, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    K577M helicase-dead mutant; S2G introduced during…

    Promoter

    CMV2

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMM288

    Plasmid

    #46036

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN E84A (WRN Human)
    

    Use

    Tre, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A exonuclease-dead mutant; S2G introduced duri…

    Promoter

    CMV2

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge