We narrowed to 22,617 results for: Ide
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Plasmid#172210PurposeFor genetic insertion in erythromycin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseGenetic insertion in prevotella copriAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only
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pUC57-Amp_CRISPIE donor B5
Plasmid#172846PurposeCRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30b_OmpAT7Sav
Plasmid#138589PurposeT7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
UseSynthetic BiologyTagsOmpA export signal and T7 tagExpressionBacterialPromoterPT7Available SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAD-sfGFP(Sp)-Pman
Plasmid#133833PurposeFluorescent protein sfGFP(Sp); under control of mannose inducible promoter.DepositorInsertsfGFP(Sp)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-tagRFP-KDEL
Plasmid#114177PurposeGateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ERDepositorInserttagRFP-KDEL
UseGateway CloningTagsKDEL retention signal and signal peptideAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 S22N
Plasmid#107106PurposeLentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Grp1-PH pET11a
Plasmid#71377PurposePhosphoinositide BindingDepositorInsertGrp1-PH (Cyth3 Mouse)
ExpressionBacterialMutationPleckstrin homology (PH) domain; aa 264-391PromoterT7Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-null
Plasmid#200182PurposeExpresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 siteDepositorInsertAAV9 Cap w/ nonsense mutation at VR8
UseAAVExpressionMammalianMutationReplaced amino acids 586-592 in Cap with nonsense…Available SinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTET2-RlucCP-3MS2
Plasmid#198354PurposePart of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterpTET2Available SinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-A
Plasmid#177784PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_class_I_1
Plasmid#164986PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genesDepositorInsertgRNA against HLA class I
UseCRISPRExpressionMammalianAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL-gRNA(anti-cGAS)-Cas9-Puro
Plasmid#130908PurposegRNA targeting human cGAS; Cas9 insert; Puromycin selection markerDepositorInsertCas9
ExpressionMammalianAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_SCP1_empty
Plasmid#99299PurposeLuciferase validation vector; empty vector without insert to determine baselineDepositorTypeEmpty backboneUseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
NPY-mApple
Plasmid#83498PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NME3
Plasmid#25536PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA FLAG mTOR ST
Plasmid#22999DepositorAvailable SinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMSCV Cyclin D2 long-wt
Plasmid#21646DepositorAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_CRF1.0
Plasmid#208655PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cellsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-roGFP2-synORP1
Plasmid#191680PurposeTi plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.DepositorInsertrredox(roGFP2-synORP1
ExpressionPlantPromoterCaMV 35SAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB2A
Plasmid#195283PurposeBicistronic DTR and Hygromycin resistance for positive/negative selectionDepositorAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only