We narrowed to 164,304 results for: HTT

1. hTLR3 flag

   Plasmid

   #13084

   Depositor

   Ruslan Medzhitov

   Insert

   TLR3 (TLR3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Amino acids 1-17 have been replaced by mammalian …

   Promoter

   Available Since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-CB-FLPe-P2A-tdTomato

   Plasmid

   #72259

   Purpose

   Express FLPe and tdTomato under CB promoter

   Depositor

   Kazuhiro Oka

   Insert

   FLPe-P2A-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T230I in tdTomato (please see depositor's co…

   Promoter

   CB promoter and chicken beta-actin + CMV enhancer

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pExp-Bla

   Plasmid

   #112561

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-Bla and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-V5-SBP-C1-HsPTB1

   Plasmid

   #64925

   Purpose

   Expresses V5- and SBP-tagged PTB1 in mammalian cells

   Depositor

   Elisa Izaurralde

   Insert

   PTB1 (PTBP1 Human)
   

   Use

   Tags

   V5 and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExp-IF2

   Plasmid

   #112567

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-IF2 and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PCDH-BCAT2

   Plasmid

   #196204

   Purpose

   overexpress BCAT2

   Depositor

   Jianjun Chen

   Insert

   BCAT2 (BCAT2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   NO

   Promoter

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ251-GERC

   Plasmid

   #47441

   Purpose

   Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.

   Depositor

   George Church

   Insert

   Gene Expression Reporter Construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4YPet-MAMM

   Plasmid

   #14032

   Depositor

   Patrick Daugherty

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

   Insert

   YPet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mammalian optimized YPet

   Promoter

   Available Since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-Trx-His6-TEV_SacB

   Plasmid

   #164588

   Purpose

   Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.

   Depositor

   Ernst Schonbrunn

   Insert

   TEV-SacB
   

   Use

   Tags

   Trx-His6 followed by TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b-ACPs (from S. pneumonia)

    Plasmid

    #63687

    Purpose

    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae

    Depositor

    Michael Johnson

    Insert

    acpS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. CAG-FLEX-MCS-WPRE-SV40 - GCH2

    Plasmid

    #230815

    Purpose

    AAV cloning vector for CRE-dependent expression under the CAG promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. R777-E202 Hs.PRKAG2-nostop

    Plasmid

    #70486

    Purpose

    Gateway ORF clone of human PRKAG2 [NM_024429.1] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PRKAG2 (PRKAG2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMVHA-E2F5

    Plasmid

    #24213

    Purpose

    Mammalian expression of human E2F5 with HA tag

    Depositor

    Kristian Helin

    Insert

    E2F5 (E2F5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. OTUD5

    Plasmid

    #28270

    Depositor

    Cheryl Arrowsmith

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    OTU DOMAIN (residues 172-339).

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pJA48 HLA-B*57:01

    Plasmid

    #180444

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*57:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.TBG.PI.FLPo.rBG

    Plasmid

    #236288

    Purpose

    AAV vector expressing FLPo from TBG promoter

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    FLPo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TBG

    Available Since

    May 20, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBS302

    Plasmid

    #11925

    Depositor

    Brian Sauer

    Article

    Sauer et al Methods Enzymol. 1993;225:890-900. doi:

    Insert

    STOP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mApple-Vinculin-N-21

    Plasmid

    #54962

    Purpose

    Localization: Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pmCherry-Paxillin

    Plasmid

    #80029

    Purpose

    Mammalian expression of mouse Paxillin

    Depositor

    Chinten James Lim

    Insert

    Paxillin (Pxn Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAG36

    Plasmid

    #35126

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    nat1
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. R777-E173 Hs.PIK3R2

    Plasmid

    #70457

    Purpose

    Gateway ORF clone of human PIK3R2 [NM_005027.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R2 (PIK3R2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGEX-2T-RhoA-wt

    Plasmid

    #12959

    Depositor

    Gary Bokoch

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pTCF37 HLA-B*35:01

    Plasmid

    #180450

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*35:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p2RZ

    Plasmid

    #27664

    Depositor

    Graeme Conn

    Article

    Walker et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Type

    Empty backbone

    Use

    Rna transcription

    Tags

    HDV Ribozyme

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RABEP1A-c019

    Plasmid

    #175477

    Purpose

    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABEP1 (RABEP1 Human)
    

    Use

    Tags

    TEV-TwinStrep

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mTurquoise-Nesprin3-C-18

    Plasmid

    #55572

    Purpose

    Localization: Nuclear Membrane , Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    Nesprin3 (SYNE3 Human)
    

    Use

    Tags

    mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

28. R777-E160 Hs.PDPK1-nostop

    Plasmid

    #70444

    Purpose

    Gateway ORF clone of human PDPK1 [NM_002613.4] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PDPK1 (PDPK1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Ptch1-mCherry

    Plasmid

    #118982

    Purpose

    Expresses mCherry tagged Ptch1 in mammalian cells

    Depositor

    Paul Hamel

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    CMV

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PS-mOrange2-MyosinIIB-C-18

    Plasmid

    #57812

    Purpose

    Localization: Cytoskeleton, Excitation: 548 / 634, Emission: 565 / 662

    Depositor

    Michael Davidson

    Insert

    MyosinIIB (MYH10 Human)
    

    Use

    Tags

    PS-mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

31. mScarletI-T2A-mTq2 pcDNA3.1

    Plasmid

    #230048

    Purpose

    Expresses mScarletI-T2A-mTq2 in human cells

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    mScarletI-T2A-mTq2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pTCF6 HLA-A*24:02

    Plasmid

    #180445

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*24:02
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. IDG_TMC7_OE_1

    Plasmid

    #161674

    Purpose

    Overexpresses TMC7

    Depositor

    Mike McManus

    Insert

    TMC7 (TMC7 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pmr101A-cPr-mCherry (KmR)

    Plasmid

    #177207

    Purpose

    Plasmid with p15A origin for constitutive mCherry expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Synthetic constitutive promoter

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTCF48 HLA-A*11:01

    Plasmid

    #180449

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*11:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRL-TK CXCR4 4x

    Plasmid

    #11307

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    4 bulged bind sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFETCh_SSRP1

    Plasmid

    #72370

    Purpose

    Donor vector for 3' FLAG tag of human SSRP1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    SSRP1 Homology Arms (SSRP1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBS xenopus Islet1 (DM#219)

    Plasmid

    #15015

    Depositor

    Douglas Melton

    Article

    Kelly et al Dev Dyn. 2000 Aug . 218(4):615-27.

    Insert

    islet1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

39. mGeos2-VEL-PMP-N-10

    Plasmid

    #57557

    Purpose

    Localization: Peroxisomes, Excitation: 503 / 569, Emission: 514 / 581

    Depositor

    Michael Davidson

    Insert

    PMP (PXMP2 Human)
    

    Use

    Tags

    mGeos2-VEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pLVX-EGFP-C3-cenexin

    Plasmid

    #73334

    Purpose

    Lentiviral vector encoding cenexin with an N-terminal EGFP tag

    Depositor

    Manuel Thery

    Insert

    cenexin/ODF2 (ODF2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge