We narrowed to 22,191 results for: his
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Plasmid#34798DepositorInsertGTP-binding protein PTD004 (OLA1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130A wgMDH
Plasmid#204266PurposeBacterial expression of R130A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130APromoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-trCIB1
Plasmid#64119PurposePhotoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1DepositorInsertdCas9-trCIB1 fusion
UseTagsHA and NLSExpressionMammalianMutationdCas9 (D10A H840A, catalytically inactive), trCIB…PromoterCMVAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-I
Plasmid#17634DepositorInsertUseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 PHR
Plasmid#160552PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 HR
UseTagsHexa hisExpressionInsectMutationPromoterAvailable SinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-STIL
Plasmid#136844PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tagDepositorInsertCLIP-STIL (STIL Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.MCV.LT339.V5.4
Plasmid#28193DepositorInsertMCPyV Truncated Tumor Large T antigen (399 tumor strain)
UseTagsV5ExpressionBacterial and MammalianMutationPromoterAvailable SinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
BacPAK Myc-S1
Plasmid#19424DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Micro
Plasmid#60410PurposeE. coli expression of SspB MicroDepositorInsertSspB micro
UseTagsHis-MBP-TEVExpressionBacterialMutationR73Q, Y11K and A15EPromoterAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
UseTagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mfn2(K109A)-16xmyc
Plasmid#26051DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L iLID (C530M)
Plasmid#60408PurposeE. coli expression of iLID (C530M)DepositorInsertiLID C530M
UseTagsHisExpressionBacterialMutationC530MPromoterAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124K wgMDH
Plasmid#204258PurposeBacterial expression of R124K wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR124KPromoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124Q wgMDH
Plasmid#204259PurposeBacterial expression of R124Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR124QPromoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124A wgMDH
Plasmid#204257PurposeBacterial expression of R124A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR124APromoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130E wgMDH
Plasmid#204267PurposeBacterial expression of R130E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130EPromoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Affibody HER2:243.BirA*-pET301/NT-DEST
Plasmid#174692PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
UseTagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationPromoterT7Available SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-AREL1
Plasmid#66710PurposeExpresses AREL1 HECT domain in E. coliDepositorAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-SCP2
Plasmid#31336DepositorInsertsterol carrier protein 2 (SCP2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Mucin MUC1-5TR-pET15b
Plasmid#174694PurposePlasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)DepositorInsertMUC1-TR
UseTagshexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
BtuF
Plasmid#92209PurposeExpression of BtuF which is the vitamin B12 binding protein for BtuCD ABC TransporterDepositorInsertBtuF (btuF )
UseTags6x His Tag and PelB Signal SequenceExpressionBacterialMutationBtuF signal sequence removed and uses the PelB si…PromoterT7 PromoterAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6A-EGFR ICD (645-1186)
Plasmid#42667DepositorInsertEGFR ICD (645-1186) (EGFR Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-TFGbeta receptor II K277R
Plasmid#11762DepositorInsertTGFbeta receptor II (TGFBR2 Human)
UseTagsHisExpressionMammalianMutationK277R: kinase deadPromoterCMVAvailable SinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
A3Bctd-max
Plasmid#198888PurposeExpress A3Bctd-max BE4max-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseTags6x His and NLSExpressionMammalianMutationPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectMutationPromoterpolyhedrinAvailable SinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcmvtag2Bmciap2 mut
Plasmid#11464DepositorAvailable SinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Centrin3
Plasmid#136806PurposeMammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrin3
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)
Plasmid#166421PurposeExpress pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)DepositorInsertNaegleria fowleri PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-YTHDF2
Plasmid#204406PurposeE. coli expression of full-length YTHDF2 with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-ZF5.3-GFP-HiBit
Plasmid#170992PurposeFor bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGRP-SA
Plasmid#21055DepositorAvailable SinceSept. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P6
Plasmid#122649PurposeExpresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla
Plasmid#84871PurposeBacterial expression of LFn-Fla fusionDepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutationPromoterT7Available SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cut-P(11)4
Plasmid#185785PurposeEncodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertCut-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only