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20,362 results

Showing: 4961 - 4980 of 20362 results

  8. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid
    #158653
    Purpose
    making transgenics expressing a UAS driven mCherry and NTR 2.0
    Depositor
    Jeff Mumm
    Publication
    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    Use
    Tol2-based transgenic expression in zebrafish/oth…
    Mutation
    F70A/F108Y
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158653-plasmid-map-sequence-id-311196

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  10. pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro

    Plasmid
    #141394
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-orf10 (ORF10 SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141394-plasmid-map-sequence-id-288099

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  13. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118154-plasmid-map-sequence-id-239465

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  16. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid
    #105539
    Purpose
    AAV expression of EGFP from hSyn promoter
    Depositor
    James M. Wilson
    Has service
    AAV1
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105539-plasmid-map-sequence-id-457689

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  19. pCW57-GFP-P2A-MCS (Neo)

    Plasmid
    #89181
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Publication
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89181-plasmid-map-sequence-id-176294

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Showing: 4961 - 4980 of 20362 results