We narrowed to 13,810 results for: OVA

Showing: 7441 - 7470 of 13810 results

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pSD141B

Plasmid

#218139

Purpose

Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56-eGFP-IRES2-mCherry

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBS U6a gRNA

Plasmid

#158961

Purpose

gRNA backbone driven by zebrafish U6a promoter

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

zf U6a promoter
gRNA backbone

Use

CRISPR

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCK083 desmaMCS:minpromEGFP,exorh:mCherry

Plasmid

#200016

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6.DC-FLAG

Plasmid

#30483

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Catalytically inactive mutant (H216/611A)

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pCB59 pDEST crybb1:mKate2

Plasmid

#195986

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pemiRFP670-tubulin

Plasmid

#136573

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP670-tubulin

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLAMP1-emiRFP670

Plasmid

#136570

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LAMP1-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-YY1

Plasmid

#104395

Purpose

HA-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1 (YY1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sasgRNACMV- mCherry-WPREpA

Plasmid

#217015

Purpose

S. aureus sgRNAs backbone along with mCherry expression

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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RNA motif plasmid cloning backbone

Plasmid

#107253

Purpose

RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

RNA motif plasmid cloning backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEFBOS_CreIRESpuro

Plasmid

#183812

Purpose

Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

Depositor

Ed Stanley

Article

Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

Insert

Cre recombinase

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-Cas9

Plasmid

#242759

Purpose

Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-Cas9

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL-3F-BirA

Plasmid

#220147

Purpose

Expresses VHL-BirA for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

codon optimized

Promoter

EF-1a

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-TBK1

Plasmid

#216709

Purpose

Mammalian expression of TBK1

Depositor

James Hurley

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

TBK1 (TBK1 Human)

Tags

TSF

Expression

Mammalian

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CamKIIa_GFP

Plasmid

#96941

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

Green fluorescent protein

Use

Lentiviral

Promoter

CaMKIIa

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST40-MFRN2

Plasmid

#50546

Purpose

Expression of MFRN2 gene in mammalian cells

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

Mrfn2 (SLC25A28 Human)

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pY095

Plasmid

#84744

Purpose

Expresses huLbCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
GFP

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pROSA26-ERT2CreERT2

Plasmid

#149436

Purpose

ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pvimentin-emiRFP703

Plasmid

#136566

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP703

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-A3-HA-ubiquitin

Plasmid

#220145

Purpose

Expresses A3-Ub for E3 substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

Ubiquitin C (UBC Human)

Tags

A3-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSD080A

Plasmid

#218138

Purpose

Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD40-eGFP-IRES2-mCherry

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCK007 pME KalTa4

Plasmid

#195965

Purpose

Gateway pME Gal4 variant

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

KalTA4 (Gal4)

Use

Other

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3

Plasmid

#90087

Purpose

Expresses wild type caspase 3 in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

Wild type

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VPR

Plasmid

#78898

Purpose

Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VPR

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

Plasmid

#175784

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

AviTag, Hexahistidine tag, and eGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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