We narrowed to 30,619 results for: ica

1. BtARR2 short

   Plasmid

   #236267

   Purpose

   Bacterial expression of bovine arrestin-2 short isoform

   Depositor

   Vsevolod Gurevich

   Insert

   arrestin-2 (ARRB2 Bovine)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   short isoform

   Promoter

   lac

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYihI-C-term K-R

   Plasmid

   #233092

   Purpose

   Expression of GST-YihI with C-term lysines (K) mutated to arginine (R)

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   GST-YihI with C-term lysines (K) mutated to arginine (R)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C-term lysines (K) mutated to arginine (R)

   Promoter

   Available since

   May 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pYihI-N-term K-R

   Plasmid

   #233091

   Purpose

   Expression of GST-YihI with N-term lysines (K) mutated to arginine (R)

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   YihI with N-term lysines (K) mutated to arginine (R)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N-terminal lysine residues mutated to arginine

   Promoter

   Available since

   May 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pRnr-S1BD

   Plasmid

   #232580

   Purpose

   Expression of the Rnr S1 and basic domains (residues 1930 to 2442) as a GST-fusion.

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   Rnr S1 + basic domain (residues 1930 to 2442)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pRnr-ND

   Plasmid

   #232579

   Purpose

   Expression of the Rnr nuclease domain (residues 649 to 1929) as a GST-fusion.

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   Rnr nuclease domain (residues 649 to 1929)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pRnr-CSD

   Plasmid

   #232578

   Purpose

   Expression of the Rnr cold shock domains I and II (residues 1 to 648) as a GST-fusion.

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   Rnr cold shock domains I and II (residues 1 to 648)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pYihI-N-term S-A

   Plasmid

   #233096

   Purpose

   Expression of GST-YihI with N-term serines (S) mutated to alanine (A)

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   YihI with N-term serines (S) mutated to alanine (A)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N-term serines (S) mutated to alanine (A)

   Promoter

   Available since

   May 7, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. ADEPT-pTarget-tetR

   Plasmid

   #238043

   Purpose

   Modified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.

   Depositor

   Lingchong You

   Article

   Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ttrB

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BtARR2 long

   Plasmid

   #236268

   Purpose

   Bacterial expression of bovine arrestin-2 long isoform

   Depositor

   Vsevolod Gurevich

   Insert

   arrestin-2 (ARRB2 Bovine)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   long isoform

   Promoter

   lac

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RNF168 C-terminal sgRNA

    Plasmid

    #207100

    Purpose

    pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GAGATGCACAAAGTAAGGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RIF C-terminal sgRNA

    Plasmid

    #207096

    Purpose

    pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AATACTAAATAGAATTTTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. REV7 C-terminal sgRNA

    Plasmid

    #207095

    Purpose

    pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GCTCATAAAGGCAGCTGAGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEVA47_dCas9-GFP_NOT_iC

    Plasmid

    #231418

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET23a-His-TEV-SpyCatcher3

    Plasmid

    #235149

    Purpose

    Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. dimerTomato-wGBD

    Plasmid

    #129635

    Purpose

    Visualization of active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    Cdc42 binding domain of N-WASP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TLCV2 - sgAP2S1 #2

    Plasmid

    #202757

    Purpose

    Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

    Depositor

    Christopher Counter

    Article

    Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

    Insert

    gRNA targeting AP2S1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TLCV2 - sgAP2S1 #1

    Plasmid

    #202756

    Purpose

    Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

    Depositor

    Christopher Counter

    Article

    Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

    Insert

    gRNA targeting AP2S1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXJ-Myc-SP5

    Plasmid

    #232653

    Purpose

    transient expresses SP5 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    SP5 (SP5 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXJ-Myc-SP2

    Plasmid

    #232652

    Purpose

    transient expresses SP2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    SP2 (SP2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

    Plasmid

    #233240

    Purpose

    Parallel coiled-coil with (GGS)3 linker to prone condensation

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vh-clover-GCN4-(GGS)3-Vt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

    Plasmid

    #233241

    Purpose

    Parallel coiled-coil with (GGS)12 linker to prevent condensation

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vh-clover-GCN4-(GGS)12-Vt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmirGLO-mir1-8mer4x (pEZ184)

    Plasmid

    #229058

    Purpose

    Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-1

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    Human miR-1 binding sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCI5-CasRx-HA (pKW88)

    Plasmid

    #229046

    Purpose

    Mammalian expression of CasRx-HA

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    CasRx (RfxCas13d)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSEVA19_sgRNA-C1_M13ps

    Plasmid

    #231411

    Purpose

    M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-C1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSEVA47_dCas9-GFP_NOT_iA

    Plasmid

    #231417

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSEVA19_sgRNA-C0_M13ps

    Plasmid

    #231410

    Purpose

    M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-C0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCN38-kimA-yfp

    Plasmid

    #227635

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    kimA-yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-AzaQ

    Plasmid

    #224429

    Purpose

    Express AzaQ in E. coli

    Depositor

    Emily Balskus

    Article

    Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

    Insert

    azaQ
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. aTY171_pPGK1_iRFP_WPRE

    Plasmid

    #225351

    Purpose

    Lentiviral vector for iRFP expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    iRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. aTY173_pPGK1_mTagBFP2_WPRE

    Plasmid

    #225352

    Purpose

    Lentiviral vector for BFP expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LifeAct-mCherry

    Plasmid

    #193723

    Purpose

    Labeling microtubules in living mammalian cells

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    LifeAct-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCN34e-yfp

    Plasmid

    #227641

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCN34e-kimA-yfp

    Plasmid

    #227638

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    kimA-yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCN38-ktrA-yfp

    Plasmid

    #227636

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    ktrA-yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTYB1-T4gp47

    Plasmid

    #228212

    Purpose

    Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

    Depositor

    Stephen Benkovic

    Article

    Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

    Insert

    47 (47 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-28a(+)-T7-OmpA-MNaseB-6xHis

    Plasmid

    #214809

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease B
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDest17_A1-LCD swap V1

    Plasmid

    #226538

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD V1

    Depositor

    Tanja Mittag

    Article

    Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

    Insert

    A1-LCD swap V1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDest17_A1-LCD swap V2

    Plasmid

    #226539

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD V2

    Depositor

    Tanja Mittag

    Article

    Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

    Insert

    A1-LCD swap V2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDest17_A1-LCD swap V3

    Plasmid

    #226540

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD V3

    Depositor

    Tanja Mittag

    Article

    Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

    Insert

    A1-LCD swap V3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDest17_A1-LCD swap X5

    Plasmid

    #226547

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD X5

    Depositor

    Tanja Mittag

    Article

    Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

    Insert

    A1-LCD swap X5
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge