We narrowed to 14,583 results for: pET

1. pET15b-Opt12

   Plasmid

   #61697

   Purpose

   Expresses PTDH mutant Opt12

   Depositor

   Huimin Zhao

   Article

   McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

   Insert

   Opt12
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-MSP1D1deltaH4/2

   Plasmid

   #71712

   Purpose

   Membrane scaffold protein (MSP), half of helix 4 truncated

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-mTDGa.1

   Plasmid

   #81051

   Purpose

   bacterial expression of catalytically dead murine TDG

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TDG (Tdg Mouse)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   asparagine 151 changed to alanine

   Promoter

   T7

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETCON-DnvLB16

   Plasmid

   #45124

   Depositor

   David Baker

   Article

   Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

   Insert

   DnvLB16
   

   Use

   Tags

   Aga2p for yeast surface display and c-myc epitope…

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-nGFPMNase

   Plasmid

   #187826

   Purpose

   express nGFPMNase protein in E coli

   Depositor

   Yikang Rong

   Article

   Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

   Insert

   nGFPMNASE
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promotor

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a AHU

   Plasmid

   #26640

   Depositor

   Phoebe Rice

   Article

   Swinger et al EMBO J. 2003 Jul 15. 22(14):3749-60.

   Insert

   AHU
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28b-NpR5600

   Plasmid

   #45741

   Depositor

   Emily Balskus, Christopher T Walsh

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   NpR5600 (Npun_R5600 Nostoc punctiforme)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-TmCA-P(11)4

   Plasmid

   #185783

   Purpose

   Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   TmCA-P(11)4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-soNadV

   Plasmid

   #83363

   Purpose

   Expresses nadV from Shewanella oneidensis in Escherichia coli

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Insert

   nadV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM41-EcPPXc

    Plasmid

    #38329

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pYpet-FsaA

    Plasmid

    #98279

    Purpose

    mammalian expression of Ypet-FsaA

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    FsaA
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET12a-AL-103 FL

    Plasmid

    #47090

    Depositor

    Marina Ramirez-Alvarado

    Article

    Martin et al Amyloid. 2010 Sep;17(3-4):129-36. doi: 1

    Insert

    AL-103 (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pET28-mHP1alpha

    Plasmid

    #181911

    Purpose

    Bacterial protein expression vector for HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET30a-YedK

    Plasmid

    #86884

    Purpose

    Bacterial expression of E. coli SRAP ortholog YedK

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    YedK (yedK Eschericia coli)
    

    Use

    Tags

    6xHis, HA tag, and S-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-cMSP26

    Plasmid

    #199769

    Purpose

    Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP26
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-cMSPdH4-6

    Plasmid

    #199766

    Purpose

    Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH46
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET29b-NpR5599

    Plasmid

    #45740

    Depositor

    Emily Balskus, Christopher T Walsh

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    NpR5599 (Npun_R5599 Nostoc punctiforme)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-hdNadV

    Plasmid

    #83362

    Purpose

    Expresses nadV from Haemophilus ducreyi in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

    Insert

    nadV
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET29a-hNABP1-C

    Plasmid

    #128311

    Purpose

    Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a Gx-NL3

    Plasmid

    #169108

    Purpose

    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28b-PU.1-ETS

    Plasmid

    #85734

    Purpose

    Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

    Depositor

    Gregory Poon

    Article

    Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

    Insert

    ETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)
    

    Use

    Tags

    6xHis following thrombin cleavage site LVPR|G

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETM41-EcLonA

    Plasmid

    #38330

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    Lon protease fragment (lon Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Polyphosphate binding fragment of Lon protease. …

    Promoter

    T7

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pDpsTE-Ypet

    Plasmid

    #98277

    Purpose

    mammalian expression of DpsTE-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Dps
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-HsSENP6

    Plasmid

    #71469

    Purpose

    Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 6 (SENP6 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pET32a-hAREG

    Plasmid

    #199229

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Amphiregulin preproprotein (AREG Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a Hbb

    Plasmid

    #26639

    Depositor

    Phoebe Rice

    Article

    Mouw et al Mol Microbiol. 2007 Mar . 63(5):1319-30.

    Insert

    Hbb (hbb Borrelia burgdorferi)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pET21a - Mtb-TF

    Plasmid

    #111513

    Purpose

    Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    Trigger Factor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Ypet-RBD

    Plasmid

    #105291

    Purpose

    To measure Rho activity by binding and FRET analysis

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    RBD (Rtkn Mouse)
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    aa encoding 7-89

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-HA-CPDSalI

    Plasmid

    #38254

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-UVRAG CC

    Plasmid

    #24400

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    UVRAG (UVRAG Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains on the CC domain, amino acids 200-270

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pET21-MRPP1_native+

    Plasmid

    #67865

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form)

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP1 (TRMT10C Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21-tVYN-Nt∆

    Plasmid

    #154431

    Purpose

    Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

    Depositor

    Ming Hammond

    Article

    Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

    Insert

    VenusΔC12-NtYcgRΔ-NLucΔ4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET51b(+)_S-Luc_CLIP

    Plasmid

    #113923

    Purpose

    Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc_CLIP
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET3_HMV-His6

    Plasmid

    #164843

    Purpose

    Expression of FL human metavinculin

    Depositor

    Tina Izard

    Article

    Rangarajan et al PLoS One. 2010 May 19;5(5):e10679. doi:

    Insert

    VCL (VCL Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PCx35IL-pET42

    Plasmid

    #42916

    Depositor

    John O'Brien

    Article

    Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

    Insert

    Connexin 35 cytoplasmic loop
    

    Use

    Tags

    6x His, GST, and S-tag

    Expression

    Bacterial

    Mutation

    cytoplasmic loop is aa 101-179

    Promoter

    T7

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET22b-HA-CPDSalI

    Plasmid

    #38253

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET30-2-GPD1L

    Plasmid

    #83912

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET23-CBD

    Plasmid

    #30311

    Depositor

    Klaus Hahn

    Insert

    CBD (WAS Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

    Plasmid

    #116966

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET31b-T7-Spinach2-Dp

    Plasmid

    #79159

    Purpose

    Expresses the Dp biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Dp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge