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We narrowed to 24,925 results for: crispr

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Showing: 481 - 500 of 24925 results

  1. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid
    #110824
    Purpose
    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2
    Depositor
    Alejandro Chavez, George Church
    Publication
    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB-MeCP2
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Promoter
    CAGGS
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19 Articles
    110824-plasmid-map-sequence-id-324008

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  5. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Publication
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    44720-plasmid-map-sequence-id-179979

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  7. pcDNA-dCas9-VP64

    Plasmid
    #47107
    Purpose
    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64
    Use
    CRISPR
    Tags
    Flag, HA, SV40 NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive)
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    47107-plasmid-map-sequence-id-398036

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  9. pcDNA-dCas9

    Plasmid
    #47106
    Purpose
    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Flag and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive)
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    47106-plasmid-map-sequence-id-433987

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  14. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Publication
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    63155-plasmid-map-sequence-id-220487

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  16. AIO-Puro

    Plasmid
    #74630
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Publication
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Puromycin resistant marker
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    74630-plasmid-map-sequence-id-373032

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  18. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    103865-plasmid-map-sequence-id-243540

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  20. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    24 Articles
    73797-plasmid-map-sequence-id-196924

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Showing: 481 - 500 of 24925 results