Addgene

We narrowed to 6,924 results for: ef1a

Showing: 481 - 500 of 6924 results

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EF1a-TET1-dCas9

Plasmid

#235593

Purpose

Tet1CD fused to N-terminus of dCas9

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)

Use

CRISPR

Tags

3xNLS, TET1 CD, and tagBFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-dCas9-VP64

Plasmid

#235595

Purpose

Expresses the dCas9-VP64 under the control of human EF1a promoter

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

dCas9-VP64

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-hfCas13d-pa

Plasmid

#233037

Purpose

To express hfCas13d from a EF1a promoter

Depositor

Jonathan Ploski

Insert

hfCas13d

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 EF1a-EGFP

Plasmid

#231925

Purpose

3rd generation lentiviral transfer plasmid with EGFP reporter gene

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_CdTag-HA

Plasmid

#221493

Purpose

NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-hP53DD (TET)

Plasmid

#214083

Purpose

Mammalian expression tetramerization domain (TET) of human TP53

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

hP53DD (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - I438M in pENTR

Plasmid

#216591

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

I438M

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - R382H in pENTR

Plasmid

#216590

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

R382H

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - G305S in pENTR

Plasmid

#216589

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

G305S

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - D252H in pENTR

Plasmid

#216588

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

D252H

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - E122K in pENTR

Plasmid

#216587

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

E122K

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - G70S in pENTR

Plasmid

#216586

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

G70S

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-HA (in pLEX_307)

Plasmid

#214918

Purpose

constitutive expression of SRRD tagged with HA

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-mXRCC1pd-eGFP

Plasmid

#206035

Purpose

Mammalian expression of PAR-deficient mXRCC1pd coupled to eGFP under the control of a EF1a promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

mXRCC1pd

Use

Tags

eGFP

Expression

Mammalian

Mutation

S105A, S186A, K188A, S195A, S221A, S222A,S238A, S…

Promoter

Ef1a

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Nde1

Plasmid

#203309

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Nhe1

Plasmid

#203310

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN1_EF1a-tNGFR-2A-Puro

Plasmid

#200553

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 1

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN2_EF1a-tNGFR-2A-Puro

Plasmid

#201112

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 2

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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