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pcDNA3.2/V5 mmu-mir-139

Plasmid

#26329

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-139 (Mir139 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (E452K)

Plasmid

#29538

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-miR106a-363

Plasmid

#19028

Depositor

Tyler Jacks

Article

Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

Insert

microRNA 106a-363 (Mir106a Mouse)

Use

Mouse Targeting

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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mCerulean3-H2B-C-10

Plasmid

#55422

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

H2B (H2BC11 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1224

Plasmid

#26337

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1224 (Mir1224 Mouse)

Expression

Mammalian

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only
pY71-tet.I.16

Plasmid

#133536

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3BP4-1/1

Plasmid

#91532

Purpose

Protein expression and purification of human SH3 domain construct SH3BP4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3BP4-1/1 (SH3BP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS2-1/3

Plasmid

#91387

Purpose

Protein expression and purification of human SH3 domain construct SORBS2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS2-1/3 (SORBS2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_VAV1-1/2

Plasmid

#91355

Purpose

Protein expression and purification of human SH3 domain construct VAV1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV1-1/2 (VAV1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRS313-PRP8

Plasmid

#90964

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Promoter

native

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAM-1/1

Plasmid

#91428

Purpose

Protein expression and purification of human SH3 domain construct STAM-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAM-1/1 (STAM Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUC 3GLA UAS HAi

Plasmid

#71763

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dSesn (1187)

Plasmid

#66919

Purpose

bacterial expression of dSesn

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn (Sesn Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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sh-Myocardin

Plasmid

#100768

Purpose

expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

sh-Myocardin

Use

RNAi

Promoter

H1

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN3-1/1

Plasmid

#91335

Purpose

Protein expression and purification of human SH3 domain construct PACSIN3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN3-1/1 (PACSIN3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum-LFA

Plasmid

#20051

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

S492A

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-RsACR_995-EYFP

Plasmid

#103772

Purpose

Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin RsACR_995

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GcACR_457-EYFP

Plasmid

#103137

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_457

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF1-ERBB4 (E563K)

Plasmid

#29541

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pOT2 3GLA

Plasmid

#71764

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MDH1-PGK-FLAG-TAT-SIVmac251

Plasmid

#35979

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

SIV strain mac251

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS426-TUB1-internal6xHis (1-440)

Plasmid

#60391

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4), amino a…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2/V5 mmu-mir-217

Plasmid

#26333

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-217 (Mir217 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
MPDZ_PDZ_7

Plasmid

#103891

Purpose

Protein expression and purification of Multiple PDZ protein PDZ7 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPDZ (MPDZ Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MPDZ_PDZ_13

Plasmid

#103897

Purpose

Protein expression and purification of Multiple PDZ protein PDZ13 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPDZ (MPDZ Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFP122

Plasmid

#21669

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
MPDZ_PDZ_3

Plasmid

#103887

Purpose

Protein expression and purification of Multiple PDZ protein PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPDZ (MPDZ Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 Budding Yeast, A. victoria)

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Available Since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K651R

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-R1ACR_741-EYFP

Plasmid

#103961

Purpose

Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_741

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA17_no stop

Plasmid

#83855

Purpose

Gateway donor vector for IAA17 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA17 (AXR3 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean3-Dectin1A-N-10

Plasmid

#55414

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2991

Plasmid

#63643

Purpose

pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKV006

Plasmid

#64764

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
A337C/Y344C

Plasmid

#24434

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pST39-His/Vps27(345-440) - Hse(275-375)

Plasmid

#17638

Depositor

James Hurley

Article

Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

Insert

His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-LYS

Plasmid

#20047

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

F489Y

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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We narrowed to 18,853 results for: REV