171,784 results

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pcDNA3.1_V5-TWIST1

Plasmid

#215030

Purpose

Mammalian expression of human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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spMSP1D1

Plasmid

#173482

Purpose

Expresses spMSP1D1 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spMSP1D1

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-tmpknot-GG-acceptor

Plasmid

#174039

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-ADAR2-sesRNA-GFP-W3SL

Plasmid

#192069

Purpose

Expression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Ctip2

Use

AAV

Expression

Mammalian

Promoter

Cbh

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FlipCherry-T2A-GFP

Plasmid

#124436

Purpose

Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipCherry-T2A-GFP

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP ZO1

Plasmid

#30313

Depositor

Alan Fanning

Article

Fanning et al FASEB J. 2002 Nov . 16(13):1835-7.

Insert

Zonula Occludens-1 (TJP1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Unique spliceform*

Available Since

Aug. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pCXLE-EGFP

Plasmid

#27082

Purpose

Integration-free (episomal) expression of EGFP

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

eGFP

Expression

Mammalian

Available Since

March 10, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEF1a-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223252

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Article

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Expression

Bacterial

Promoter

sppA

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

Plasmid

#158988

Purpose

Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-KRAB

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Expression

Worm

Promoter

tag-168

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCE-hOCT3/4

Plasmid

#41813

Purpose

Non-integrating (episomal) expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV_ENVLPE+

Plasmid

#232427

Purpose

Expression plasmid to produce ENVLPE+ particles

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ENVLPE+

Expression

Mammalian

Promoter

2xCMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244084

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Golgi-7

Plasmid

#55052

Purpose

Localization: Golgi Complex, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Golgi (B4GALT1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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