We narrowed to 9,322 results for: control

1. INCTbiosyn-pAct-AtINT4

   Plasmid

   #127530

   Purpose

   Plasmid encodes A. thaliana codon optimized Integrase 4.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 4 coding sequence codon optimized for A. thaliana expression.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3

   Plasmid

   #135564

   Purpose

   An AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporter

   Depositor

   Christopher Richie

   Article

   Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

   Inserts

   miR-30 FF3
   Nuc-EYFP
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   SYN1 and hSYN1

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-RIPK1 K163R

   Plasmid

   #78844

   Purpose

   To overexpress RIPK1 K163R in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K163R

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCLASP_Crz1(19A)_CLASP_RGS2membrane

   Plasmid

   #133085

   Purpose

   Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   Crz1*-CLASP
   

   Expression

   Bacterial and Yeast

   Promoter

   pADH1

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. J23114-mRFP1-331Bb

   Plasmid

   #78279

   Purpose

   mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   J23114-mRFP1
   

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ULK2-K39I

   Plasmid

   #97076

   Purpose

   Expresses mutant ULK2 in mammalian cells

   Depositor

   Pasquale Vito

   Article

   Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

   Insert

   ULK2 (ULK2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Lysine 39 to Isoleucine

   Promoter

   CMV

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5 GST MetaP2

   Plasmid

   #112750

   Purpose

   express HA-GST-FRB-MetaP2

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   HA-GST-FRB-MetaP2 (METAP2 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-3F2-mCherry

   Plasmid

   #73418

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3F2 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNAS1b split mCherry NEDD4 WW2 pos ctrl

   Plasmid

   #112029

   Purpose

   Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Insert

   NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
   

   Tags

   Split mCherry

   Expression

   Bacterial

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mito-yHS1-M7A,H102A

    Plasmid

    #159165

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A,H102A
    

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGEX-4T-1-RIPK3 4KR

    Plasmid

    #78828

    Purpose

    To purify GST-tagged recombinant RIPK3 4KR protein

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K55R, K89R, K363R, K501R

    Promoter

    tac

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJET-49-DExogron-mNeonGreen-R11a

    Plasmid

    #179903

    Purpose

    Donor with homologous arms for Rab11a to knock in DExogron (=minilAA7-DExCon) mNeonGreen module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    minilAA7-mNeonGreen

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3GS

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIBN-tagBFP-hNCL

    Plasmid

    #103805

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hNCL (CIB1, NCL Mustard Weed, Human)
    

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid

    #165492

    Purpose

    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    

    Use

    AAV

    Tags

    darkVenus

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2-PcyA-IRES-HO1

    Plasmid

    #131787

    Purpose

    Tol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PcyA and HO1
    

    Use

    Synthetic Biology; Zebrafish tol2 transposon

    Tags

    MTS (Mitochondrial Targeting Signal from subunit …

    Expression

    Mammalian

    Promoter

    hsp70

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti PA-mCit-NL

    Plasmid

    #113452

    Purpose

    Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    hUBbC

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p1.2-GS

    Plasmid

    #162770

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV ShadowG-AURKA-mTurq2

    Plasmid

    #157768

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC1534 pscAAV mU6 shRNA(PKCd) CMV-IE Nuc-EYFP

    Plasmid

    #135562

    Purpose

    An AAV vector expressing shRNA vs rat PKCd and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    shRNA (mouse PKCd)
    Nuc-EYFP
    

    Use

    AAV and RNAi

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    CMV-IE and mU6

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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