We narrowed to 27,291 results for: Tat

1. pET-DEUT RUVBL1 Delete

   Plasmid

   #159144

   Purpose

   Expression and protien purification of mutated RUVBL1

   Depositor

   Eric Erquan Zhang

   Article

   Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

   Insert

   RUVBL1 (RUVBL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acid 1-463, Glu134-Glu237 mutated to GPPG

   Promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tom20-mChF-AIP(TE)

   Plasmid

   #61522

   Purpose

   Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutation

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   FK506 binding protein 1A (FKBP1A Human)
   

   Use

   Tags

   AIP with TE mutation (see comments), Tom20, and m…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCR8/GW/TOPO-ELK3

   Plasmid

   #98622

   Purpose

   Gateway entry TOPO TA cloning vector for ELK3

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK3 (Elk3 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   E167K

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG CMV14 / BRAF-V600K

   Plasmid

   #131720

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600K

   Promoter

   CMV

   Available since

   Dec. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3xFLAG-CMV TIP60 212-364

   Plasmid

   #78795

   Purpose

   To overexpress TIP60 212-364 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

   Insert

   TIP60 (KAT5 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   212-364, G214A

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1_SNORD27h_2ntmut

   Plasmid

   #73067

   Purpose

   expression clone for human mutated SNORD27 (C/D box snoRNA U27)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD27 (E2F7 Human)
   

   Use

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   2 nt mutation in AS box that binds E2F7 pre-mRNA

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-HA-BHLHE40(S243A/E248A)

   Plasmid

   #110155

   Purpose

   Expression of human BHLHE40 (S243A/E248A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S243A/E248A

   Promoter

   CMV

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-shMBNL3-0455

   Plasmid

   #115463

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shMBNL3-0455 (MBNL3 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV V5-TbID-LBR

   Plasmid

   #175100

   Purpose

   Lentiviral expression of V5-TbID-tagged mouse Lbr

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Lbr (Lbr Mouse)
   

   Use

   Lentiviral

   Tags

   V5-TbID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMCSF-R(mA)-luc

    Plasmid

    #12421

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    C/EBP binding site mutation (created a KpnI site …

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-CMV-dSa VP64 Neurog2

    Plasmid

    #99695

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPC-L647RLaminA-CS

    Plasmid

    #69062

    Purpose

    encodes a lamin A mutant with Leucine 647 mutated to an Arginin and with a substitution of cysteine in CaaX-motif by a serine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    LaminA (LMNA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Lamin A with Leucine 647 mutated to Arginine and …

    Promoter

    CMV

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLV-Hyg-Snrnp40-CRISPR-resistant

    Plasmid

    #134251

    Purpose

    Lentivector encoding CRISPR-resistant Snrnp40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mutated coding sequence “gataactatgcgacgttgaa” to…

    Promoter

    EF1a

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-AR-R405S

    Plasmid

    #126051

    Purpose

    Expresses human androgen receptor (R405S) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R405S

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_RBM45_WT

    Plasmid

    #82892

    Purpose

    Gateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    RBM45 (RBM45 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-myc-Sema4D-CD4-FLAG

    Plasmid

    #51601

    Purpose

    expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    Sema4D/CD4-FLAG (Sema4d Mouse, Human)
    

    Use

    Tags

    FLAG and myc

    Expression

    Mammalian

    Mutation

    Sema4D amino acids 660-861 were replaced with CD4…

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

    Plasmid

    #184407

    Purpose

    yeast surface display of the SARS-CoV-2 Delta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    L452R+T478K

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p426_Cas9_gRNA-R-ARS607c

    Plasmid

    #87409

    Purpose

    p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3xFLAG CMV14 / BRAF-D594E

    Plasmid

    #131714

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D594E

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR8/GW/TOPO-ELF1

    Plasmid

    #98627

    Purpose

    Gateway entry TOPO TA cloning vector ELF1

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELF1 (Elf1 Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. INSR gRNA (BRDN0001145804)

    Plasmid

    #75506

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human INSR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    INSR (INSR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-cAICDA-BE3

    Plasmid

    #113422

    Purpose

    Expresses cAICDA-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    cAICDA-BE3 (aicda Ictalurus punctatus (channel catfish))
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330-Esrrb gRNA

    Plasmid

    #128841

    Purpose

    gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Esrrb gRNA (Esrrb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TGFBR2 gRNA (BRDN0001146812)

    Plasmid

    #77468

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TGFBR2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TGFBR2 (TGFBR2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCEP4-myc-CXCR4-W161Y

    Plasmid

    #98951

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    W161Y: enhanced CXCL12 binding and signaling

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCEP4-myc-CXCR4-W161H

    Plasmid

    #98952

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    W161H: enhanced CXCL12 binding and signaling

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TWF1 gRNA (BRDN0001149022)

    Plasmid

    #77287

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TWF1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TWF1 (TWF1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX-GST-CHD5(Chromos)-FRAG

    Plasmid

    #68875

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only the two chromodomains of CHD5

    Promoter

    tac

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pRS416 ATG5-E141D-HA-ATG12-ATG16

    Plasmid

    #166851

    Purpose

    Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.

    Depositor

    Daniel Klionsky

    Article

    Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

    Insert

    ATG5 ATG12 ATG16 (ATG5, ATG12, ATG16 Budding Yeast)
    

    Use

    Tags

    HA-ATG12

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TAF1 gRNA (BRDN0001145911)

    Plasmid

    #77870

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TAF1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TAF1 (TAF1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLKO.1-shMBNL3-9465

    Plasmid

    #115464

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL3-9465 (MBNL3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMY-Ly6G-P2A-PuroR-HA-Rluc_miR

    Plasmid

    #163340

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. DYRK1B gRNA (BRDN0001146749)

    Plasmid

    #76622

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYRK1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DYRK1B (DYRK1B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSLIK-Neo-TGmiR-Gb2

    Plasmid

    #25746

    Purpose

    Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Gb2 (Gnb2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCEP4-myc-CXCR4-H79Q

    Plasmid

    #98953

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant H79Q

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    H79Q: enhanced CXCL12 binding and signaling

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Chicken ArcLight S174

    Plasmid

    #53567

    Purpose

    Genetically encoded voltage sensor ArcLight with improved kinetics

    Depositor

    Vincent Pieribone

    Article

    Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

    Insert

    Chicken ArcLight-S174
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gg-VSP contains an R153Q mutation and an amino ac…

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p3xFLAG-CMV TIP60 390-513

    Plasmid

    #78797

    Purpose

    To overexpress TIP60 390-513 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    TIP60 (KAT5 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    aa390-513

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pCDF1-GRM3 (S610L)

    Plasmid

    #31800

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed serine 610 to leucine

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pCDF1-GRM3 (E870K)

    Plasmid

    #31802

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed glutamic acid 870 to lysine

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pZS1[GalS-T,RbsR-T]

    Plasmid

    #60773

    Purpose

    Contains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    GalS-T
    GalS-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. TAN DBD with GalS LB…

    Promoter

    Available since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge