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We narrowed to 18,759 results for: nls

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Showing: 10041 - 10080 of 18759 results

  3. pMOD_A1710

    Plasmid
    #91039
    Purpose
    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-P2A-TaCas9_H840A nickase
    Use
    CRISPR
    Mutation
    H840A
    Promoter
    ZmUbi
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91039-plasmid-map-sequence-id-181793

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  6. pDIRECT_26G

    Plasmid
    #91149
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91149-plasmid-map-sequence-id-182283

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  7. pDIRECT_21B

    Plasmid
    #91129
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91129-plasmid-map-sequence-id-182252

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  9. pMOD_A0202

    Plasmid
    #91003
    Purpose
    Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AtCas9_D10A nickase
    Use
    CRISPR
    Mutation
    D10A
    Promoter
    AtUbi10
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91003-plasmid-map-sequence-id-181759

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  10. pY122 (pcDNA3.1-huCPbCpf1)

    Plasmid
    #92298
    Purpose
    Expression of humanized CPbCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huCPbCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92298-plasmid-map-sequence-id-181648

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  11. pY118 (pcDNA3.1-huLb4Cpf1)

    Plasmid
    #92294
    Purpose
    Expression of humanized Lb4Cpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huLb4Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92294-plasmid-map-sequence-id-181497

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  12. FUCas9Cyan

    Plasmid
    #85554
    Purpose
    Expresses Cas9 with fluorescent Cyan reporter
    Depositor
    Marco Herold
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanised S.pyrogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    hUBC
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85554-plasmid-map-sequence-id-180892

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  14. pTX208

    Plasmid
    #89268
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02
    Depositor
    Daniel Voytas
    Publication
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsYSA-gRNA01 and OsYSA-gRNA02
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Promoter
    Maize Ubiquitin1 promoter
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89268-plasmid-map-sequence-id-177893

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  16. p426_Cas9_gRNA-ARS911b

    Plasmid
    #87394
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS911b sequence GTAATATTGTCTTGTTTCCC in yeast chromosome 9.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS911b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87394-plasmid-map-sequence-id-193437

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  17. p426_Cas9_gRNA-ARS1309a

    Plasmid
    #87399
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1309a sequence CCTGTGGTGACTACGTATCC in yeast chromosome 13.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1309a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87399-plasmid-map-sequence-id-508300

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  18. p425_Cas9_gRNA_LEU_1014a

    Plasmid
    #87407
    Purpose
    p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87407-plasmid-map-sequence-id-193449

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  22. pY036_ATP1A1_G5

    Plasmid
    #86618
    Purpose
    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86618-plasmid-map-sequence-id-168853

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  23. pLNT-SFFV-mRuby2

    Plasmid
    #87217
    Purpose
    lentiviral expression of mRuby2
    Depositor
    Violaine Sée
    Insert
    mRuby2
    Use
    Lentiviral
    Tags
    6xHis-T7
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    87217-plasmid-map-sequence-id-173864

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  25. pX330-sgRNA_Dicer_3

    Plasmid
    #68809
    Purpose
    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.
    Depositor
    Constance Ciaudo
    Publication
    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA mouse Dicer1
    Use
    CRISPR and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    68809-plasmid-map-sequence-id-120931

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  31. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  32. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid
    #75400
    Purpose
    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    hCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    35S
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75400-plasmid-map-sequence-id-146044

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  34. pKT3Ts-goldyTALEN

    Plasmid
    #80330
    Purpose
    Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence
    Depositor
    Jeffrey Essner
    Publication
    Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi
    Type
    Empty backbone
    Use
    TALEN
    Expression
    Bacterial
    Promoter
    T3
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80330-plasmid-map-sequence-id-156328

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  39. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid
    #74603
    Purpose
    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry
    Depositor
    Ron Vale
    Publication
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    SFFV
    Available since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74603-plasmid-map-sequence-id-141268

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Showing: 10041 - 10080 of 18759 results