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pVY-19 TC-sfGFP-tag3

Plasmid

#194633

Purpose

fluorescent protein with cationic peptide tag and tetracysteine tag

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-sfGFP-K(GGSKKRKKR)3

Tags

KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-15 TC-CAT-tag3

Plasmid

#194629

Purpose

antibiotic resistance enzyme with cationic peptide tag and tetracysteine tag

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-CAT-(GGSKKRKKR)3

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Zic4-3505:Luc

Plasmid

#193002

Purpose

Plasmid to test the Hydra Zic4 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al bioRxiv 2021.12.22.47383

Insert

Hydra Zic4 promoter

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2094

Plasmid

#162142

Purpose

Complementation plasmid for MlaE E98A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(E98A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2095

Plasmid

#162143

Purpose

Complementation plasmid for MlaE K205A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(K205A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2097

Plasmid

#162144

Purpose

omplementation plasmid for MlaE D250A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(D250A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2057

Plasmid

#162145

Purpose

Expression plasmid for MlaE Tyr81Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Tyr81Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2060

Plasmid

#162146

Purpose

Expression plasmid for MlaE Val77Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Val77Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2061

Plasmid

#162147

Purpose

Expression plasmid for MlaE Leu78Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Leu78Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2062

Plasmid

#162148

Purpose

Expression plasmid for MlaE Leu70Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Leu70Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2063

Plasmid

#162149

Purpose

Expression plasmid for MlaE Leu99Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Leu99Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2065

Plasmid

#162150

Purpose

Expression plasmid for MlaE Trp149Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Trp149Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2066

Plasmid

#162151

Purpose

Expression plasmid for MlaE Phe209Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Phe209Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2139

Plasmid

#162132

Purpose

Expression and Complementation plasmid for MlaD TM swap with LptC TM

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(TMdeltaLptC TM)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2138

Plasmid

#162133

Purpose

Expression and Complementation plasmid for MlaD TM swap with LetB TM

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(TMdeltaLetB TM)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1198

Plasmid

#162134

Purpose

Expression and complementation plasmid for MlaFEDCB, with N-terminal his tag on MlaE

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2093

Plasmid

#162135

Purpose

Expression and Complementation plasmid for MlaE IF1 1-15 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-15)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2132

Plasmid

#162136

Purpose

Expression and Complementation plasmid for MlaE IF1 1-25 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-25)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2092

Plasmid

#162137

Purpose

Expression and Complementation plasmid for MlaE IF1 1-30 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-30)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2133

Plasmid

#162138

Purpose

Expression and Complementation plasmid for MlaE IF1 1-39 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-39)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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