We narrowed to 10,928 results for: cat.1
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Plasmid#211632PurposesgRNA-1 against ERCC6LDepositorInsertsgRNA ERCC6L
UseCRISPRExpressionMammalianPromoterU6Available SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Δ1~34,Δ215~284-TwStrep
Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HiGlpG- S116A, H196A
Plasmid#158539PurposeBacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196ADepositorInsertHiGlpG
TagsGST tagExpressionBacterialMutationS116A;H169AAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMPO 51
Plasmid#68358PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2). ApR, expression vector with rrnBT1T2-Pm-MCSII, ColE1 replication originDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1 Clover-LMNA Donor
Plasmid#122508PurposeHomology repair template for in frame (first exon) clover knock-in of human LMNA geneDepositorInsertHomology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
UseHomology repair template plasmid (donor plasmid)TagsCloverAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PARP2
Plasmid#175810PurposepENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal epitope tagsDepositorAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::LEU2
Plasmid#227594PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::LEU2
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::URA3
Plasmid#227595PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::URA3
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::HIS3
Plasmid#227596PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::HIS3
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::TRP1
Plasmid#227593PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::TRP1
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-1_enzymatic-domain
Plasmid#183385PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attR3
Plasmid#213054PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3DepositorInsertsgRNA
ExpressionBacterialAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOGG005
Plasmid#113980PurposeLevel 1 high copy number vector for E. coli expressionDepositorTypeEmpty backboneExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG026
Plasmid#113992PurposeLevel 1 golden gate cloning vector for low copy number stable environmental gene expressionDepositorTypeEmpty backboneExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG024
Plasmid#113991PurposeLevel 1 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABL2
Plasmid#39178PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MALT1 (3K0W)
Plasmid#25306PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmalt1.129.326 (MALT1 Human)
TagsHisExpressionBacterialMutationamino acid residues 128-326Available SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
MALT1 (3BFO)
Plasmid#25216PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmalt1.226.314 (MALT1 Human)
TagsHisExpressionBacterialMutationContains amino acid residues 226-314 of Isoform 2Available SinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Venus-Bik-G154STOP-pEGFP-C1
Plasmid#177428PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
TagsVenusExpressionMammalianMutationGlycine 154 replaced with STOP codonPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PRKAA1
Plasmid#23871DepositorInsertPRKAA1 (PRKAA1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-deltaH-AMBRA1-3xFLAG
Plasmid#174158PurposeVector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)DepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
MALT1-DD (2G7R)
Plasmid#25613PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 31, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS416 CUP1pro-Htt103QdeltaP125-134-YFP
Plasmid#224882PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-134 (HTT Human)
TagsYFPExpressionYeastMutationdeltaP125-134PromoterCUP1Available SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP135-170-YFP
Plasmid#224878PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP135-170 (HTT Human)
TagsYFPExpressionYeastMutationdeltaP135-170PromoterCUP1Available SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-160-YFP
Plasmid#224886PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-160 (HTT Human)
TagsYFPExpressionYeastMutationdeltaP141-160PromoterCUP1Available SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-160-YFP
Plasmid#224883PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-160 (HTT Human)
TagsYFPExpressionYeastMutationdeltaP125-160PromoterCUP1Available SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-170-YFP
Plasmid#224880PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-170 (HTT Human)
TagsYFPExpressionYeastMutationdeltaP141-170PromoterCUP1Available SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP161-170-YFP
Plasmid#224876PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.DepositorInsertHTT103QdeltaP161-170 (HTT Human)
TagsYFPExpressionYeastMutationdelta161-170PromoterCUP1Available SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9-X1.1
Plasmid#196836Purposenon-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV9-X1.1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV1.X1
Plasmid#209780Purposenon-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV1-X1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin
Plasmid#231549PurposeMammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarizationDepositorAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMVtrunc msfGFPΔC10-beta-actin
Plasmid#231548PurposeMammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopyDepositorAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A_mmFbxw7(res) (closed)
Plasmid#160105PurposeExpresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNADepositorAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmFbxw7(res)
Plasmid#160106PurposeExpresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNADepositorAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-GIT2 [N83/48R]
Plasmid#140079PurposeMammalian Expression Plasmid of anti-GIT2 (Human). Derived from hybridoma N83/48.DepositorInsertanti-GIT2 (Human) recombinant mouse monoclonal antibody (GIT2 Mouse)
ExpressionMammalianPromoterdual CMVAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-RH▲-MNase
Plasmid#136292PurposeExpression of catalytic inactive RNase H fused to micrococcal nucleaseDepositorInsertRNase HI-D10R-E48R and MNase
ExpressionBacterialAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mouse PKA-Calpha-mEGFP
Plasmid#45521DepositorInsertPKA catalytic subunit alpha tagged by mEGFP
ExpressionMammalianAvailable SinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mouse PKA-Calpha-mPAGFP
Plasmid#45524DepositorInsertPKA catalytic subunit alpha tagged by mPAGFP
ExpressionMammalianAvailable SinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK DPC4 deltaC Flag
Plasmid#12628DepositorInsertDPC4 delta C (SMAD4 Human)
TagsflagExpressionMammalianMutationC-terminal truncated DPC4 (aa 1-511)Available SinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only