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pUC-GFP-HS

Plasmid

#133305

Purpose

"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

hok/sok toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDule2-para-aminoPhe

Plasmid

#85503

Purpose

Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

Insert

p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Tags

None

Expression

Bacterial

Mutation

Y32T, E107T, D158P, I159L, L162A

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1

Plasmid

#166823

Purpose

Gateway pDONR vector containing NLRP1 (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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NET5s pEGFP-N2 (1154)

Plasmid

#62034

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET5s (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

short form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-Myc-IRES-GFP

Plasmid

#35396

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
GFP

Use

Retroviral

Expression

Mammalian

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

Plasmid

#218767

Purpose

encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-pAAFRS-9

Expression

Bacterial

Mutation

Y37G, L71V, D182C, F183Y, L186C, D265R

Promoter

tacI

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-Optimized E6AP

Plasmid

#165100

Purpose

ecoli optimized expression of GST-fusion of human E6AP

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2013 Apr 12;288(15):10349-6

Insert

E6AP isoform 3 (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-pcDNA3.1

Plasmid

#85760

Purpose

mammalian expression of full length HA tagged USPL1

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length (USPL1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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NET5L pEGFP-N2 (1155)

Plasmid

#62035

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

NET5L (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

long form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
Stat1 alpha pRC/CMV

Plasmid

#8690

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 alpha (STAT1 Human)

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pDONR P2R-P3-EYFP

Plasmid

#48351

Purpose

Contributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EYFP

Use

Gateway entry vector

Mutation

Contains a stop codon.

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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Stat1 beta Flag pRc/CMV

Plasmid

#8704

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 beta (STAT1 Human)

Tags

Flag Tag

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-DEST47-SIRT1

Plasmid

#242131

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT1 (SIRT1 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p8325 pLIX YAP1 WT

Plasmid

#184527

Purpose

Expression of YAP1 WT

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 WT (YAP1 Human)

Use

Lentiviral

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF5B

Plasmid

#134625

Purpose

Mammalian expression of mouse KIF5B 376-963

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCI H2B-GFP

Plasmid

#92399

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

IRES H2B EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGIPz-mPD-L1 3SA

Plasmid

#121489

Purpose

Expression of mouse PD-L1 3SA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1 3SA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S175A, T179A, S183A

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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