We narrowed to 15,565 results for: LEA

1. pET21-pelB-LaG-24

   Plasmid

   #172750

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-24
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-9

   Plasmid

   #172742

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-9
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-SRAP

   Plasmid

   #84524

   Purpose

   Expresses murine SRAP domain (250 aa) in bacteria

   Depositor

   Douglas Feldman

   Article

   Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

   Insert

   SRAP domain (Hmces Mouse)
   

   Use

   Tags

   6xHis and S-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. cycLuc_SbMVs

   Plasmid

   #119209

   Purpose

   Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   cyclic luciferase with SbMVp cleavage site
   

   Use

   Luciferase

   Tags

   AU1 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xEGFP-mMeikin (E156R, R159E)

   Plasmid

   #174710

   Purpose

   Mammalian expression of mouse Meikin with Separase-resistant mutations

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin (Meikin Mouse)
   

   Use

   Tags

   3xEGFP

   Expression

   Mammalian

   Mutation

   E156R, R159E

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNOC-CRISPR-35S

   Plasmid

   #99861

   Purpose

   Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET41a iGluf

   Plasmid

   #119833

   Purpose

   Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluf
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E25D

   Promoter

   T7

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBCV

   Plasmid

   #62659

   Purpose

   Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   FLPo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-V5-Vrk2

   Plasmid

   #120240

   Purpose

   Expresses V5-tagged Vrk2 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Vrk2 (Vrk2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIPZ shCUX1-5328

    Plasmid

    #100815

    Purpose

    Lentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099

    Depositor

    Alain Nepveu

    Article

    Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

    Insert

    TGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CaMKIIa-ChRger3-TS-EYFP

    Plasmid

    #127241

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger3-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAimR

    Plasmid

    #98687

    Purpose

    Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

    Depositor

    Rotem Sorek

    Article

    Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

    Insert

    AimR
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Native

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7 shRNA against CRTC1

    Plasmid

    #72710

    Purpose

    Lentiviral expression of shRNA against CRTC1 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC1
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pRSFDuet-1_VNp-DARPinOFF7

    Plasmid

    #182397

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pResQ shDna2'

    Plasmid

    #31952

    Depositor

    Sheila Stewart

    Article

    Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

    Insert

    shRNA against hDna2 (DNA2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pICSL11023

    Plasmid

    #117542

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S_OMEGA(pICH51288)

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IBMc097

    Plasmid

    #161945

    Purpose

    Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge