We narrowed to 8,323 results for: gnal

1. NES-EGFP-cPHx2

   Plasmid

   #116854

   Purpose

   PI(3,4)P2 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amino acids 169-329 (tandem dimer)

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194377

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Human, Synthetic)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-hPhyB621-mCherry-HRasCT

   Plasmid

   #100282

   Purpose

   Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-621 aa) (PHYB Mustard Weed)
   

   Use

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-621 aa)

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rab8-T22N(Flag) in pcDNA3.1neo

   Plasmid

   #46784

   Purpose

   expression of T22N Rab8a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB8A T22N (RAB8A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T22N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pEF073 MBP-Axin-His

   Plasmid

   #154011

   Purpose

   Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   Axin1 (AXIN1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos3.2FP

   Plasmid

   #203757

   Purpose

   Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   NRas c-term (NRAS Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM60-FLAG-SLC38A9.1 (1-119aa)

   Plasmid

   #71862

   Purpose

   lentiviral stable expression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized, first 119 amino acids

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGEX-4T1-WDR5

   Plasmid

   #59969

   Purpose

   Bacterial Expression GST-WDR5

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

9. myc-Atg14 (396-492)

   Plasmid

   #86755

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   atg14 (ATG14 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   aa 396-492

   Promoter

   CMV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. myc-Atg14 (181-413)

    Plasmid

    #86754

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 181-413

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. myc-Atg14 (201-492)

    Plasmid

    #86751

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 201-492

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGem-nHyPer:cAPX

    Plasmid

    #84736

    Purpose

    Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    APX1 (APX1 Mustard Weed)
    HyPer2 targeted to nucleus
    

    Use

    Tags

    NLS-SV40

    Expression

    Plant

    Mutation

    Promoter

    pFMV/p35S

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCLXSN-Tax

    Plasmid

    #44038

    Depositor

    Shao-Cong Sun

    Article

    Rivera-Walsh et al J Biol Chem. 2001 Nov 2;276(44):40385-8.

    Insert

    5'UTR-Tax-3'UTR (CNTN2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCMV/myc/ER-αT2ib

    Plasmid

    #110769

    Purpose

    Expresses ER intrabody against mouse and human TLR2

    Depositor

    Thomas Böldicke

    Article

    Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

    Insert

    intrabody against mouse and human TLR2
    

    Use

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCINeo-mCHMP5

    Plasmid

    #11771

    Depositor

    Sankar Ghosh

    Article

    Shim et al J Cell Biol. 2006 Mar 27. 172(7):1045-56

    Insert

    chromatin modifying protein 5 (Chmp5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

16. eGFP Depdc5 pLJM1

    Plasmid

    #46380

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Promoter

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBBA57-TEV-His12

    Plasmid

    #137033

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length BBA57, contains signal pep…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid

    #87827

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CFAP410_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176948

    Purpose

    Mammalian expression vector encoding CFAP410 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CFAP410 (C21orf2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-JAK1Gly590Arg

    Plasmid

    #174573

    Purpose

    Human FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    JAK1Gly590Arg
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Gly590Arg

    Promoter

    CMV

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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