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Showing: 5121 - 5140 of 167150 results

  3. pFLAG_WT MDA5

    Plasmid
    #216798
    Purpose
    Expresses wild type (wt) melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Expression
    Mammalian
    Available Since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216798-plasmid-map-sequence-id-428831

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  14. pLV6-Bmal-luc

    Plasmid
    #68833
    Purpose
    Expression of Bmal1-luc in mammalian cells
    Depositor
    Steven Brown
    Insert
    Bmal1 regulatory sequences (Bmal1 Mouse)
    Use
    Lentiviral and Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68833-plasmid-map-sequence-id-455401

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  15. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

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  18. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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  19. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter and IRES
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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Showing: 5121 - 5140 of 167150 results