Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 156,062 results for: ins

Showing: 7681 - 7710 of 156062 results

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pOmnibac_ZZ_linker_K560_GFP_SNAP

Plasmid

#159720

Purpose

Expresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (only amino acids 1-560), K560 (KIF5B Human)

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Mutation

Only amino acids 1-560

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC39A9_STOP

Plasmid

#161355

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A9 (SLC39A9 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC16A11_STOP

Plasmid

#161105

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A11 (SLC16A11 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC6A18_STOP

Plasmid

#161385

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A18 (SLC6A18 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC6A17_STOP

Plasmid

#161373

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A17 (SLC6A17 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC6A15_STOP

Plasmid

#161043

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A15 (SLC6A15 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC6A20_STOP

Plasmid

#161409

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A20 (SLC6A20 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

Plasmid

#200977

Purpose

Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

D62Q-Q130L-V167L (increase specificity towards he…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

Plasmid

#200980

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

Plasmid

#217798

Purpose

Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR and Lentiviral

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

EF1a

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC35C1_STOP

Plasmid

#161229

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35C1 (SLC35C1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-TNT-CFP-STIM1-C227W

Plasmid

#68403

Purpose

Expresses a STIM1 gain-of-function mutant in mammalian cells

Depositor

Yubin Zhou

Article

Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

Insert

CFP-STIM1 (STIM1 Human)

Tags

Cyan Fluorescent Protein (CFP)

Expression

Mammalian

Mutation

Changed Cysteine 227 to Tryptophan; CFP was inser…

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC13A2_STOP

Plasmid

#161087

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC13A2 (SLC13A2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC30A3_STOP

Plasmid

#161267

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A3 (SLC30A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC14A2_STOP

Plasmid

#161431

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC14A2 (SLC14A2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDam-Myc-HP1

Plasmid

#59221

Purpose

expresses Dam-myc-HP1

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Insert

HP1 (Su(var)205 Fly)

Use

Damid

Tags

Dam and myc

Expression

Insect

Promoter

Drosophila heat shock promoter

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-SPNS3_STOP

Plasmid

#161451

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SPNS3 (SPNS3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC5A7_STOP

Plasmid

#161372

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A7 (SLC5A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-NIPAL3_STOP

Plasmid

#161457

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NIPAL3 (NIPAL3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-NIPAL2_STOP

Plasmid

#161445

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NIPAL2 (NIPAL2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX RASGAP(N)-SH2

Plasmid

#46432

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(N) (RASA1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 168-283

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC22A11_STOP

Plasmid

#161129

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A11 (SLC22A11 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC22A1_STOP

Plasmid

#161200

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A1 (SLC22A1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX SHP-2(C)-SH2

Plasmid

#46497

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(C) (PTPN11 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 101-222

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC15A2_STOP

Plasmid

#161052

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC15A2 (SLC15A2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC22A12_STOP

Plasmid

#161141

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A12 (SLC22A12 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC30A8_STOP

Plasmid

#161302

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A8 (SLC30A8 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

Plasmid

#221894

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

EWSR1 (EWSR1 Human)

Tags

6xHis-MBP-PreScission and TEV-mGFP

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC6A7_STOP

Plasmid

#161374

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A7 (SLC6A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-SLC7A10_STOP

Plasmid

#161398

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A10 (SLC7A10 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

Showing: 7681 - 7710 of 156062 results

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