We narrowed to 40,900 results for: Eras
-
Plasmid#112031PurposeMode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)DepositorInsert14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
TagsSplit mCherryExpressionBacterialAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0374 (His-MBP-Cas8_Cas7_Cas6)
Plasmid#130639PurposeExpresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.DepositorInsertVchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on Cas8ExpressionBacterialPromoterT7Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBV-luc TOP2A 1kb promoter
Plasmid#138274PurposeLuciferase reporter plasmid driven by TOP2A promoterDepositorInsertTOP2A promoter sequence
UseLuciferaseExpressionMammalianAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-TGFBR2-wt_3'UTR
Plasmid#31882DepositorInsertTGFBR2 wild-type 3'UTR (TGFBR2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterSV40Available SinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-Mettl4
Plasmid#86665PurposeExpresses murine Mettl4 containing N-terminal 3XFlag tagDepositorAvailable SinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127315PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
Tags3xFLAG and CL1/PESTExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
Tags3xFLAG and PESTExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV Reep3-V5
Plasmid#175119PurposeLentiviral expression of mouse Reep3-V5DepositorAvailable SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
LB124 (Bcl-2 from -1287 to -3934)
Plasmid#15240DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from -1287 to -3934 with P1Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long UTR
Plasmid#21649DepositorAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem9-V5
Plasmid#175151PurposeLentiviral expression of mouse Tmem9-V5DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p4xSMAD_RE
Plasmid#118051PurposeGBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motifDepositorInsert4 copies the SMAD DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…ExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2xp53_RE
Plasmid#118053PurposeGBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
KCC2B_HUMAN_D0
Plasmid#79718PurposeThis plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2B
TagsHis10-TEVExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
LMP FFB
Plasmid#89491PurposeFirefly hairpinDepositorInsertFirefly luciferase hairpin, clone B
UseMouse Targeting and RetroviralExpressionMammalianPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available SinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MK14_HUMAN_D0
Plasmid#79680PurposeThis plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK14
TagsHis10-TEVExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmbim6-V5
Plasmid#175150PurposeLentiviral expression of mouse Tmbim6-V5DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
LB335 (Bcl-2 from ATG to -1281)
Plasmid#15382DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -1281 with P2Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (Frag B)
Plasmid#16606DepositorInsertc-myc promoter (Frag B) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationFragB (KpnI->AccI). The KpnI site is at -1194 …Available SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (Frag C)
Plasmid#16607DepositorInsertc-myc promoter (Frag C) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationFragC (AccI->PvuII). The AccI site is at -741 …Available SinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-950 to -700)
Plasmid#66133PurposeLuciferase-based reporter for VEGF promoter region (-950 to -700 bp)DepositorInsertVascular Endothelial Growth Factor-A promoter region (VEGFA Human)
UseLuciferasePromoterVEGF -950 to -700Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
Tagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1Available SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
J-HARS-4
Plasmid#166529PurposescFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-SARS-7
Plasmid#166527PurposescFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-RARS-5
Plasmid#166535PurposescFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-KARS-5
Plasmid#166530PurposescFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-DARS-1
Plasmid#166538PurposescFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-LARS-15
Plasmid#166542PurposescFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LB1366 (Bcl-2 promoter with MHS1234)
Plasmid#15388DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS1234Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS186
Plasmid#140627PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
UseCRISPR; TransposonExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only