We narrowed to 11,588 results for: EMB

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pL2-GGA(EAAAK)2AGG

Plasmid

#137104

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGA(EAAAK)2AGG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-CD86-mApple

Plasmid

#133860

Purpose

Mammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Tags

EGFP and mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPagP

Plasmid

#112125

Purpose

Bacterial expression of outer membrane protein PagP, wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

PagP (pagP E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_1

Plasmid

#124912

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Arl6ip6

Plasmid

#120235

Purpose

Expresses V5-tagged Arl6ip6 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Arl6ip6 (Arl6ip6 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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GST-RP2

Plasmid

#86072

Purpose

A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPc

Plasmid

#87166

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPc

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tacδ

Plasmid

#81177

Purpose

Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tacδ

Tags

KLGFFKR

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 WT

Plasmid

#66139

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pGS1011

Plasmid

#63783

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

Strep-tag II followed by TRP1 selection marker

Use

E. coli cloning vector

Tags

Strep-tag II

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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TAPBPL pEGFP-C3 (1765)

Plasmid

#62055

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

TAPBPL (TAPBPL Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
mCherry_LD 0_CS A_tTA-BFP

Plasmid

#58873

Purpose

MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_CS M_tTA-BFP

Plasmid

#58874

Purpose

MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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Bambi NGFR

Plasmid

#44628

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Bambi (Bambi Mouse)

Use

Retroviral

Tags

IRES and truncated NGFR

Expression

Mammalian

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only
TAL2387

Plasmid

#36796

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CYLD-TALEN_Right (Cyld Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only