Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 25,138 results for: crispr

Clear keywords
Showing: 5141 - 5160 of 25138 results

  1. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Publication
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Human, Synthetic, S. Pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165602-plasmid-map-sequence-id-327024

    Enlarge

  2. P628_SpCas9-RecA

    Plasmid
    #87263
    Purpose
    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency
    Depositor
    Yonglun Luo
    Publication
    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RecA
    Use
    CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    no
    Promoter
    CBh
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87263-plasmid-map-sequence-id-173851

    Enlarge

  8. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161815-plasmid-map-sequence-id-320654

    Enlarge

  9. pV1200

    Plasmid
    #111429
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Publication
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111429-plasmid-map-sequence-id-212464

    Enlarge

  15. pFETCh_ATF4

    Plasmid
    #72350
    Purpose
    Donor vector for 3' FLAG tag of human ATF4
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATF4 Homology Arms (ATF4 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72350-plasmid-map-sequence-id-147903

    Enlarge

  20. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

    Enlarge

Showing: 5141 - 5160 of 25138 results