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We narrowed to 18,247 results for: multi

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Showing: 5161 - 5180 of 18247 results

  3. pLM-AMA18.0 dCas9-VPR

    Plasmid
    #138945
    Purpose
    Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138945-plasmid-map-sequence-id-366628

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  4. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid
    #83107
    Purpose
    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide
    Depositor
    Andrew Brooks
    Inserts
    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    Use
    Lentiviral
    Tags
    2A self cleaving peptide
    Expression
    Mammalian
    Mutation
    R24C and p64 isoform
    Promoter
    hPGK and hPGK (single transcript)
    Available since
    July 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83107-plasmid-map-sequence-id-330062

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  5. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid
    #26797
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Expresses tTA under EF1α promoter separately.
    Depositor
    Edward Hsiao
    Publication
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Rs1 (HTR4 Human)
    tTA
    Tags
    mCherry-P2A
    Expression
    Mammalian
    Mutation
    D100A
    Promoter
    EF1α and short TetO, miniCMV
    Available since
    Feb. 23, 2011
    Availability
    Academic Institutions and Nonprofits only
    26797-plasmid-map-sequence-id-12685

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  8. pCAGGS-mTagBFP2-T2A-iCre

    Plasmid
    #113837
    Purpose
    Monomeric TagBFP2 and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCre
    Expression
    Mammalian
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113837-plasmid-map-sequence-id-222246

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  9. pU6_HEK3+1T>A_(PP7-C4-Q1)

    Plasmid
    #232437
    Purpose
    pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus
    Depositor
    Gil Westmeyer
    Publication
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    232437-plasmid-map-sequence-id-462756

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  11. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Publication
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164559-plasmid-map-sequence-id-330205

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  13. pCOM-CD63-COM

    Plasmid
    #138350
    Purpose
    Expressing COM-CD63-COM fusion protein in mammalian cells
    Depositor
    Baisong Lu
    Publication
    Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12
    Insert
    COM-CD63-COM fusion protein
    Use
    CRISPR and Lentiviral
    Tags
    COM is fused to the N and C termini of human CD63
    Expression
    Mammalian
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    138350-plasmid-map-sequence-id-359687

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  15. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid
    #117274
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
    Depositor
    David Stephens
    Publication
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    Tags
    mScarlet-i
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV and IRES
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    117274-plasmid-map-sequence-id-233028

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  16. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid
    #117720
    Purpose
    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA
    Depositor
    Joerg Mansfeld
    Publication
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    mAID-nanobody
    IBB-mCherry
    Tags
    importin-beta bining domain (IBB) and triple HA
    Expression
    Mammalian
    Mutation
    codon optimized mAID for murine expression
    Promoter
    CMV and None, IRES-driven
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117720-plasmid-map-sequence-id-227032

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  20. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Promoter
    pdx
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67279-plasmid-map-sequence-id-115888

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Showing: 5161 - 5180 of 18247 results