Addgene

We narrowed to 904 results for: TRP1

Showing: 751 - 780 of 904 results

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Aga2p-LOV-Turbo1-myc_pCTCON2

Plasmid

#199673

Purpose

expresses pre-evolved version of LOV-Turbo on the yeast surface

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo1

Use

Tags

Aga2p and Myc

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA2-GAE

Plasmid

#199175

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA2-GAE domain (GGA2 Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Contains Lys 545 Gln substitution

Promoter

ADH1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA3-GAE

Plasmid

#199176

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA3-GAE domain (GGA3 Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-γ2 ear

Plasmid

#199178

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ2 ear domain (AP1G2 Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA1-GAE

Plasmid

#199174

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA1-GAE domain (GGA1 Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-AAGAB

Plasmid

#198332

Purpose

Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Use

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Use

Tags

Cmyc and HA

Expression

Yeast

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700]-Ss(424)

Plasmid

#166852

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

pCu

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 L306P

Plasmid

#169262

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 L306P (NOP4 Budding Yeast)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu306Pro)

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 WT

Plasmid

#169265

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 WT (RBM28 Human)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 L351P

Plasmid

#169266

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 L351P (RBM28 Human)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu351Pro)

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

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pRR-AR

Plasmid

#73045

Purpose

Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

Depositor

David McMillen

Article

Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

Insert

Androgen receptor (AR Human)

Use

Tags

Expression

Yeast

Mutation

please see depositor comments below

Promoter

glyceraldehyde phosphate dehydrogenase (GPD)

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pNubWtXgate

Plasmid

#105079

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Use

Tags

NubI

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJS646

Plasmid

#183175

Purpose

Y2H vector compatible with pJS647 PYR1 insert

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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VEGF binding scFv G6

Plasmid

#111716

Purpose

Yeast Surface Display of G6 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6

Use

Tags

cmyc tag

Expression

Yeast

Mutation

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCashGem-Xyl2-URA3-LINEAR

Plasmid

#174840

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Mutation

Promoter

ENO1p

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pUDC175

Plasmid

#103019

Purpose

S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Use

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pXNgate22-1HA

Plasmid

#105077

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Use

Tags

NubG

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pXNubWtgate

Plasmid

#105080

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Use

Tags

NubI

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pXNgate21-3HA

Plasmid

#105078

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Use

Tags

NubG

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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KM-CashGem-Xyl2-URA3-LINEAR

Plasmid

#174838

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Mutation

Promoter

TEF1p

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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YL-CashGem-BAT1-URA3-LINEAR

Plasmid

#174837

Purpose

pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Mutation

Promoter

GPDp

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook2

Plasmid

#198524

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook2 (HOOK2 Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

His488Gln substitution

Promoter

ADH1

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDN-G1Tt

Plasmid

#44509

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR

Use

Synthetic Biology; Expression regulator/reporter

Tags

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Use

Tags

cmyc tag

Expression

Yeast

Mutation

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

Plasmid

#225228

Purpose

Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (25Q)

Use

Tags

HA Tag and c-myc Tag

Expression

Yeast

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

Plasmid

#225229

Purpose

Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (46Q)

Use

Tags

HA Tag and c-myc Tag

Expression

Yeast

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pOBD2

Plasmid

#196826

Purpose

Yeast two-hybrid ; Gal4 DNA binding domain

Depositor

Stan Fields

Article

Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

Type

Empty backbone

Use

Tags

Gal4 DNA binding domain

Expression

Yeast

Mutation

Promoter

ADH

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-γ1 ear

Plasmid

#199177

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ1 ear domain (Ap1g1 Mouse)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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