Addgene

We narrowed to 6,033 results for: WUS;

Showing: 751 - 780 of 6033 results

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-2.5Kb MMP10

Plasmid

#53973

Purpose

Luciferase reporter for mouse MMP10 2.5kb promoter

Depositor

Michael Chin

Article

Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

Insert

mouse MMP10 2.5Kb promoter (Mmp10 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PCR from C57BL6/J

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/EGFP/pdCas9-C1

Plasmid

#162759

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (S366A)

Plasmid

#214163

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (S366A) (STING1 Human)

Use

Retroviral

Tags

Expression

Mutation

S366A

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (R232)

Plasmid

#214162

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVTAG-NEMOL329P

Plasmid

#11971

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Leucine329 to Proline

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
SL2-sgSat-MTSb/BFP/pdCas9-C1

Plasmid

#162761

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting centromeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgSat-MTSb)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-NEMOD311N

Plasmid

#11968

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOD311N (IKBKG Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Asp311 to Asn

Promoter

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV-FlagBcl10K31RK63R

Plasmid

#18719

Depositor

Jon Ashwell

Article

Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

B cell lymphoma 10 (BCL10 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Lysine 31 to arginine and Lysine 63 to arginine.

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-NEMOL329P

Plasmid

#11967

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Leucine329 to Proline

Promoter

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pN1-mCherry-NKD2

Plasmid

#176641

Purpose

Expresses NKD2-mCherry fusion protein in mammalian cells

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

mCherry tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET-NEMOL329P

Plasmid

#11969

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Use

Tags

His

Expression

Bacterial

Mutation

Leucine329 to Proline

Promoter

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2AB

Plasmid

#13475

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2AB (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-136

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1DCH2B

Plasmid

#13471

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2B (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2B; Delta 47-137

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2A

Plasmid

#13473

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-46

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2B

Plasmid

#13474

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2B (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 47-136

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A

Plasmid

#13476

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L17A

Plasmid

#13477

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L17A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 17 to Alanine

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pLei-sfGFP-D134*

Plasmid

#191110

Purpose

An E. coli sfGFP reporter with one TAG at position 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Use

Tags

His tag

Expression

Bacterial

Mutation

changing D134 to TAG

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*

Plasmid

#191111

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Use

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 to TAG

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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3H-α-synuclein

Plasmid

#205714

Purpose

Mammalian expression of 3H-alpha-synuclein

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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α-synuclein-3H

Plasmid

#205715

Purpose

Mammalian expression of alpha-synuclein-3H

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-αS-2

Plasmid

#205706

Purpose

Mammalian expression of alpha-synuclein tandem dimer

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein tandem dimer (SNCA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#1

Plasmid

#174323

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hNKD2

Plasmid

#174329

Purpose

encodes human NKD2 cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

MycHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Thy1.1 DEST

Plasmid

#17442

Purpose

Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#186452

Purpose

used as source for acquisition of new spacers

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-DNAJC5

Plasmid

#205727

Purpose

Mammalian expression of DNAJC5

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pME-p3E-HyPer7

Plasmid

#194289

Purpose

Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (S366A)

Plasmid

#214155

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (S366A) (STING1 Human)

Use

Tags

Expression

Mammalian

Mutation

S366A

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-GBA-N370S-nick sgRNA

Plasmid

#214096

Purpose

Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G13A nicking sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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